Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation.
Published
Journal Article
We describe an engineered fluorescent optogenetic sensor, SuperClomeleon, that robustly detects inhibitory synaptic activity in single, cultured mouse neurons by reporting intracellular chloride changes produced by exogenous GABA or inhibitory synaptic activity. Using a cell-free protein engineering automation methodology that bypasses gene cloning, we iteratively constructed, produced, and assayed hundreds of mutations in binding-site residues to identify improvements in Clomeleon, a first-generation, suboptimal sensor. Structural analysis revealed that these improvements involve halide contacts and distant side chain rearrangements. The development of optogenetic sensors that respond to neural activity enables cellular tracking of neural activity using optical, rather than electrophysiological, signals. Construction of such sensors using in vitro protein engineering establishes a powerful approach for developing new probes for brain imaging.
Full Text
Duke Authors
Cited Authors
- Grimley, JS; Li, L; Wang, W; Wen, L; Beese, LS; Hellinga, HW; Augustine, GJ
Published Date
- October 9, 2013
Published In
Volume / Issue
- 33 / 41
Start / End Page
- 16297 - 16309
PubMed ID
- 24107961
Pubmed Central ID
- 24107961
Electronic International Standard Serial Number (EISSN)
- 1529-2401
Digital Object Identifier (DOI)
- 10.1523/JNEUROSCI.4616-11.2013
Language
- eng
Conference Location
- United States