Distinct polyadenylation landscapes of diverse human tissues revealed by a modified PA-seq strategy.

Published online

Journal Article

BACKGROUND: Polyadenylation is a key regulatory step in eukaryotic gene expression and one of the major contributors of transcriptome diversity. Aberrant polyadenylation often associates with expression defects and leads to human diseases. RESULTS: To better understand global polyadenylation regulation, we have developed a polyadenylation sequencing (PA-seq) approach. By profiling polyadenylation events in 13 human tissues, we found that alternative cleavage and polyadenylation (APA) is prevalent in both protein-coding and noncoding genes. In addition, APA usage, similar to gene expression profiling, exhibits tissue-specific signatures and is sufficient for determining tissue origin. A 3' untranslated region shortening index (USI) was further developed for genes with tandem APA sites. Strikingly, the results showed that different tissues exhibit distinct patterns of shortening and/or lengthening of 3' untranslated regions, suggesting the intimate involvement of APA in establishing tissue or cell identity. CONCLUSIONS: This study provides a comprehensive resource to uncover regulated polyadenylation events in human tissues and to characterize the underlying regulatory mechanism.

Full Text

Duke Authors

Cited Authors

  • Ni, T; Yang, Y; Hafez, D; Yang, W; Kiesewetter, K; Wakabayashi, Y; Ohler, U; Peng, W; Zhu, J

Published Date

  • September 11, 2013

Published In

Volume / Issue

  • 14 /

Start / End Page

  • 615 -

PubMed ID

  • 24025092

Pubmed Central ID

  • 24025092

Electronic International Standard Serial Number (EISSN)

  • 1471-2164

Digital Object Identifier (DOI)

  • 10.1186/1471-2164-14-615

Language

  • eng

Conference Location

  • England