Mitigation of variation observed in a peripheral blood mononuclear cell (PBMC) based HIV-1 neutralization assay by donor cell pooling.

Published

Journal Article

Cultured primary peripheral blood mononuclear cells (PBMC) represent a potentially physiologic in vitro model of HIV-1 infection, but assessment of antibody-mediated HIV-1 neutralization using PBMC has been hindered by donor variability and lack of a sustainable individual PBMC source. To advance this model for HIV vaccine evaluation, intra- and inter-assay variability were assessed using monoclonal and polyclonal antibodies and PBMC targets from multiple HIV-seronegative donors. Inter-assay variability was introduced by using different PBMC for virus propagation, and more substantially, for assay targets. Neutralization titers varied by as much as 4 logs when using different individual donor PBMC as targets; variability was antibody-specific, with the greatest variation observed using an individual polyclonal plasma. Pooling of multiple PBMC donors significantly reduced median inter-assay variation to the level of intra-assay variation, suggesting a pathway forward for establishing a uniform, sustainable and standardized approach to the assessment of antibody function using a PBMC model.

Full Text

Duke Authors

Cited Authors

  • Wieczorek, L; Brown, BK; Delsarto Macedo, C; Wesberry-Schmierer, M; Ngauy, V; Rosa Borges, A; Michael, NL; Marovich, MA; Montefiori, DC; Polonis, VR

Published Date

  • December 2013

Published In

Volume / Issue

  • 447 / 1-2

Start / End Page

  • 240 - 248

PubMed ID

  • 24210120

Pubmed Central ID

  • 24210120

Electronic International Standard Serial Number (EISSN)

  • 1096-0341

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2013.09.014

Language

  • eng

Conference Location

  • United States