Persistently adenovirus-infected lymphoid cells express microRNAs derived from the viral VAI and especially VAII RNA.

Published

Journal Article

Human adenovirus can establish latent infections in lymphoid tissues in vivo and persistent, infections in cultured lymphoid cell lines. During lytic infection, adenovirus expresses microRNAs (miRNAs) derived from the viral non-coding RNAs VAI and, especially, VAII. Here, we demonstrate that persistently adenovirus-infected human BJAB cells also produce adenovirus-derived miRNAs primarily derived from the viral VAII RNA, which contributes ~2.7% of all RNA-induced silencing complex (RISC)-associated RNAs. However, our data indicate that the 5' end of the predominant VAII-derived viral RNA, and hence its seed sequence, differs from what has been previously reported. Our data demonstrate that adenovirus expresses viral miRNAs in chronically infected lymphoid cells and raise the possibility that these may contribute to the maintenance of the latently adenovirus-infected lymphoid cells previously observed in mucosal-associated lymphoid tissues in vivo.

Full Text

Duke Authors

Cited Authors

  • Furuse, Y; Ornelles, DA; Cullen, BR

Published Date

  • December 2013

Published In

Volume / Issue

  • 447 / 1-2

Start / End Page

  • 140 - 145

PubMed ID

  • 24210108

Pubmed Central ID

  • 24210108

Electronic International Standard Serial Number (EISSN)

  • 1096-0341

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2013.08.024

Language

  • eng

Conference Location

  • United States