Proteomic analysis of pulmonary edema fluid and plasma in patients with acute lung injury.


Journal Article

Proteomics is the large-scale analysis of protein profiles. This approach has not yet been reported in the study of acute lung injury (ALI). This study details protein profiles in plasma and pulmonary edema fluid (EF) from 16 ALI patients and plasma and bronchoalveolar lavage fluid (BALF) from 12 normal subjects. More than 300 distinct protein spots were evident in the EF and BALF of both normal subjects and ALI patients. Of these, 158 were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In the plasma and EF protein profile of ALI patients, there were multiple qualitative changes. For instance, in all normal subjects, but in only one of the ALI patients, seven distinct surfactant protein A isoforms were evident. Nearly all ALI patients also had protein spots that indicate truncation or other posttranslational modifications. Several of these novel changes could serve as new biomarkers of lung injury.

Full Text

Cited Authors

  • Bowler, RP; Duda, B; Chan, ED; Enghild, JJ; Ware, LB; Matthay, MA; Duncan, MW

Published Date

  • June 2004

Published In

Volume / Issue

  • 286 / 6

Start / End Page

  • L1095 - L1104

PubMed ID

  • 14742308

Pubmed Central ID

  • 14742308

Electronic International Standard Serial Number (EISSN)

  • 1522-1504

International Standard Serial Number (ISSN)

  • 1040-0605

Digital Object Identifier (DOI)

  • 10.1152/ajplung.00304.2003


  • eng