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Structure of hamster insulin: comparison with a tumor insulin

Publication ,  Journal Article
Neelon, FA; Delcher, HK; Steinman, H; Lebovitz, HE
Published in: Federation Proceedings
January 1, 1973

Insulin, isolated from hamster pancreata by acid ethanol extraction, membrane ultrafiltration, DEAE cellulose and Sephadex G50 chromatography, was pure and used for sequence studies. Amino acid sequence was established by tryptic cleavage and both manual and automatic Edman degradation. The proposed B chain sequence is identical with that of rabbit insulin, while A chain is identical to that of rat. However, this specific combination of A and B chains is unique. An insulin immunoreactive peptide was isolated from a transplantable hamster pancreatic carcinoma by a similar process. Chromatographic behavior was identical to that of normal hamster insulin but the tumor preparation was contaminated with peptides which were only removed after sulfitolysis of the tumor insulin to respective A and B chains. Amino acid composition and partial sequence data show that the tumor product is identical to the normal pancreatic insulin.

Duke Scholars

Published In

Federation Proceedings

ISSN

0014-9446

Publication Date

January 1, 1973

Volume

32

Issue

3 (I)

Start / End Page

476

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Neelon, F. A., Delcher, H. K., Steinman, H., & Lebovitz, H. E. (1973). Structure of hamster insulin: comparison with a tumor insulin. Federation Proceedings, 32(3 (I)), 476.
Neelon, F. A., H. K. Delcher, H. Steinman, and H. E. Lebovitz. “Structure of hamster insulin: comparison with a tumor insulin.” Federation Proceedings 32, no. 3 (I) (January 1, 1973): 476.
Neelon FA, Delcher HK, Steinman H, Lebovitz HE. Structure of hamster insulin: comparison with a tumor insulin. Federation Proceedings. 1973 Jan 1;32(3 (I)):476.
Neelon, F. A., et al. “Structure of hamster insulin: comparison with a tumor insulin.” Federation Proceedings, vol. 32, no. 3 (I), Jan. 1973, p. 476.
Neelon FA, Delcher HK, Steinman H, Lebovitz HE. Structure of hamster insulin: comparison with a tumor insulin. Federation Proceedings. 1973 Jan 1;32(3 (I)):476.

Published In

Federation Proceedings

ISSN

0014-9446

Publication Date

January 1, 1973

Volume

32

Issue

3 (I)

Start / End Page

476

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 1116 Medical Physiology
  • 0606 Physiology
  • 0601 Biochemistry and Cell Biology