High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma.
Journal Article (Journal Article)
A rapid, selective and reproducible high-performance liquid chromatographic method for the analysis in plasma of the calcium channel blocking agent, diltiazem, and one of its metabolites, deacetyldiltiazem is described. The method involves extraction with the methyl tert.-butyl ether of the drugs and the internal standard (verapamil), back-extraction into sulphuric acid and reversed-phase chromatography with UV detection. Over a concentration range of 10-1000 ng/ml the average coefficient of variation for diltiazem was 5.4% and for deacetyldiltiazem was 8.3%.
Full Text
Duke Authors
Cited Authors
- Verghese, C; Smith, MS; Aanonsen, L; Pritchett, EL; Shand, DG
Published Date
- January 14, 1983
Published In
- J Chromatogr
Volume / Issue
- 272 / 1
Start / End Page
- 149 - 155
PubMed ID
- 6841534
Digital Object Identifier (DOI)
- 10.1016/s0378-4347(00)86111-3
Language
- eng
Conference Location
- Netherlands