High-performance liquid chromatographic analysis of diltiazem and its metabolite in plasma.

Journal Article (Journal Article)

A rapid, selective and reproducible high-performance liquid chromatographic method for the analysis in plasma of the calcium channel blocking agent, diltiazem, and one of its metabolites, deacetyldiltiazem is described. The method involves extraction with the methyl tert.-butyl ether of the drugs and the internal standard (verapamil), back-extraction into sulphuric acid and reversed-phase chromatography with UV detection. Over a concentration range of 10-1000 ng/ml the average coefficient of variation for diltiazem was 5.4% and for deacetyldiltiazem was 8.3%.

Full Text

Duke Authors

Cited Authors

  • Verghese, C; Smith, MS; Aanonsen, L; Pritchett, EL; Shand, DG

Published Date

  • January 14, 1983

Published In

  • J Chromatogr

Volume / Issue

  • 272 / 1

Start / End Page

  • 149 - 155

PubMed ID

  • 6841534

Digital Object Identifier (DOI)

  • 10.1016/s0378-4347(00)86111-3


  • eng

Conference Location

  • Netherlands