Autophagy is redundant for the host defense against systemic Candida albicans infections.

Journal Article

Autophagy has been demonstrated to play an important role in the immunity against intracellular pathogens, but very little is known about its role in the host defense against fungal pathogens such as Candida albicans. Therefore, the role of autophagy for the host defense against C. albicans was assessed by complementary approaches using mice defective in autophagy, as well as immunological and genetic studies in humans. Although C. albicans induced LC3-II formation in macrophages, myeloid cell-specific ATG7(-/-) mice with defects in autophagy did not display an increased susceptibility to disseminated candidiasis. In in vitro experiments in human blood mononuclear cells, blocking autophagy modulated cytokine production induced by lipopolysaccharide, but not by C. albicans. Furthermore, autophagy modulation in human monocytes did not influence the phagocytosis and killing of C. albicans. Finally, 18 single-nucleotide polymorphisms in 13 autophagy genes were not associated with susceptibility to candidemia or clinical outcome of disease in a large cohort of patients, and there was no correlation between these genetic variants and cytokine production in either candidemia patients or healthy controls. Based on these complementary in vitro and in vivo studies, it can be concluded that autophagy is redundant for the host response against systemic infections with C. albicans.

Full Text

Duke Authors

Cited Authors

  • Smeekens, SP; Malireddi, RK; Plantinga, TS; Buffen, K; Oosting, M; Joosten, LAB; Kullberg, BJ; Perfect, JR; Scott, WK; van de Veerdonk, FL; Xavier, RJ; van de Vosse, E; Kanneganti, T-D; Johnson, MD; Netea, MG

Published Date

  • May 2014

Published In

Volume / Issue

  • 33 / 5

Start / End Page

  • 711 - 722

PubMed ID

  • 24202731

Electronic International Standard Serial Number (EISSN)

  • 1435-4373

Digital Object Identifier (DOI)

  • 10.1007/s10096-013-2002-x

Language

  • eng

Conference Location

  • Germany