Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae.
Journal Article (Journal Article)
In the yeast Saccharomyces cerevisiae and most other eukaryotes, mitotic recombination is important for the repair of double-stranded DNA breaks (DSBs). Mitotic recombination between homologous chromosomes can result in loss of heterozygosity (LOH). In this study, LOH events induced by ultraviolet (UV) light are mapped throughout the genome to a resolution of about 1 kb using single-nucleotide polymorphism (SNP) microarrays. UV doses that have little effect on the viability of diploid cells stimulate crossovers more than 1000-fold in wild-type cells. In addition, UV stimulates recombination in G1-synchronized cells about 10-fold more efficiently than in G2-synchronized cells. Importantly, at high doses of UV, most conversion events reflect the repair of two sister chromatids that are broken at approximately the same position whereas at low doses, most conversion events reflect the repair of a single broken chromatid. Genome-wide mapping of about 380 unselected crossovers, break-induced replication (BIR) events, and gene conversions shows that UV-induced recombination events occur throughout the genome without pronounced hotspots, although the ribosomal RNA gene cluster has a significantly lower frequency of crossovers.
Full Text
Duke Authors
Cited Authors
- Yin, Y; Petes, TD
Published Date
- October 2013
Published In
Volume / Issue
- 9 / 10
Start / End Page
- e1003894 -
PubMed ID
- 24204306
Pubmed Central ID
- PMC3814309
Electronic International Standard Serial Number (EISSN)
- 1553-7404
Digital Object Identifier (DOI)
- 10.1371/journal.pgen.1003894
Language
- eng
Conference Location
- United States