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Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae.

Publication ,  Journal Article
Yin, Y; Petes, TD
Published in: PLoS Genet
October 2013

In the yeast Saccharomyces cerevisiae and most other eukaryotes, mitotic recombination is important for the repair of double-stranded DNA breaks (DSBs). Mitotic recombination between homologous chromosomes can result in loss of heterozygosity (LOH). In this study, LOH events induced by ultraviolet (UV) light are mapped throughout the genome to a resolution of about 1 kb using single-nucleotide polymorphism (SNP) microarrays. UV doses that have little effect on the viability of diploid cells stimulate crossovers more than 1000-fold in wild-type cells. In addition, UV stimulates recombination in G1-synchronized cells about 10-fold more efficiently than in G2-synchronized cells. Importantly, at high doses of UV, most conversion events reflect the repair of two sister chromatids that are broken at approximately the same position whereas at low doses, most conversion events reflect the repair of a single broken chromatid. Genome-wide mapping of about 380 unselected crossovers, break-induced replication (BIR) events, and gene conversions shows that UV-induced recombination events occur throughout the genome without pronounced hotspots, although the ribosomal RNA gene cluster has a significantly lower frequency of crossovers.

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Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

October 2013

Volume

9

Issue

10

Start / End Page

e1003894

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Polymorphism, Single Nucleotide
  • Mitosis
  • Loss of Heterozygosity
  • Genome, Fungal
  • Gene Conversion
  • Developmental Biology
  • DNA Replication
 

Citation

APA
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MLA
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Yin, Y., & Petes, T. D. (2013). Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. PLoS Genet, 9(10), e1003894. https://doi.org/10.1371/journal.pgen.1003894
Yin, Yi, and Thomas D. Petes. “Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae.PLoS Genet 9, no. 10 (October 2013): e1003894. https://doi.org/10.1371/journal.pgen.1003894.
Yin, Yi, and Thomas D. Petes. “Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae.PLoS Genet, vol. 9, no. 10, Oct. 2013, p. e1003894. Pubmed, doi:10.1371/journal.pgen.1003894.

Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

October 2013

Volume

9

Issue

10

Start / End Page

e1003894

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Saccharomyces cerevisiae
  • Recombination, Genetic
  • Polymorphism, Single Nucleotide
  • Mitosis
  • Loss of Heterozygosity
  • Genome, Fungal
  • Gene Conversion
  • Developmental Biology
  • DNA Replication