Exome sequencing identifies distinct mutational patterns in liver fluke-related and non-infection-related bile duct cancers.

Journal Article (Journal Article)

The impact of different carcinogenic exposures on the specific patterns of somatic mutation in human tumors remains unclear. To address this issue, we profiled 209 cholangiocarcinomas (CCAs) from Asia and Europe, including 108 cases caused by infection with the liver fluke Opisthorchis viverrini and 101 cases caused by non-O. viverrini-related etiologies. Whole-exome sequencing (n = 15) and prevalence screening (n = 194) identified recurrent somatic mutations in BAP1 and ARID1A, neither of which, to our knowledge, has previously been reported to be mutated in CCA. Comparisons between intrahepatic O. viverrini-related and non-O. viverrini-related CCAs demonstrated statistically significant differences in mutation patterns: BAP1, IDH1 and IDH2 were more frequently mutated in non-O. viverrini CCAs, whereas TP53 mutations showed the reciprocal pattern. Functional studies demonstrated tumor suppressive functions for BAP1 and ARID1A, establishing the role of chromatin modulators in CCA pathogenesis. These findings indicate that different causative etiologies may induce distinct somatic alterations, even within the same tumor type.

Full Text

Duke Authors

Cited Authors

  • Chan-On, W; Nairismägi, M-L; Ong, CK; Lim, WK; Dima, S; Pairojkul, C; Lim, KH; McPherson, JR; Cutcutache, I; Heng, HL; Ooi, L; Chung, A; Chow, P; Cheow, PC; Lee, SY; Choo, SP; Tan, IBH; Duda, D; Nastase, A; Myint, SS; Wong, BH; Gan, A; Rajasegaran, V; Ng, CCY; Nagarajan, S; Jusakul, A; Zhang, S; Vohra, P; Yu, W; Huang, D; Sithithaworn, P; Yongvanit, P; Wongkham, S; Khuntikeo, N; Bhudhisawasdi, V; Popescu, I; Rozen, SG; Tan, P; Teh, BT

Published Date

  • December 2013

Published In

Volume / Issue

  • 45 / 12

Start / End Page

  • 1474 - 1478

PubMed ID

  • 24185513

Electronic International Standard Serial Number (EISSN)

  • 1546-1718

Digital Object Identifier (DOI)

  • 10.1038/ng.2806


  • eng

Conference Location

  • United States