Stage-specific and differential notch dependency at the alphabeta and gammadelta T lineage bifurcation.
Journal Article (Journal Article)
Signals transduced by Notch receptors are indispensable for T cell specification and differentiation of alphabeta T lineage cells. However, the role of Notch signals during alphabeta versus gammadelta T lineage decision remains controversial. Here, we addressed this question by employing a clonal analysis of CD4(-)CD8(-) (DN) progenitor potential to position the divergence of alphabeta and gammadelta T cell lineages to the late DN2 to DN3 developmental stages. Accordingly, alphabeta and gammadelta precursor frequencies within these T cell progenitor subsets were determined, both in the presence and absence of Notch signaling through Delta-like 1. Notch signals were found to be critical for the DN to CD4(+)CD8(+) (DP) transition, irrespective of the identity (pTalphabeta or gammadelta) of the inducing T cell receptor complex, whereas gammadelta T cells developed from gammadeltaTCR-expressing T cell progenitors in the absence of further Notch ligand interaction. Collectively, our findings demonstrate a differential, stage-specific requirement for Notch receptor-ligand interactions in the differentiation of alphabeta and gammadelta T cells from T cell progenitors.
Full Text
Duke Authors
Cited Authors
- Ciofani, M; Knowles, GC; Wiest, DL; von Boehmer, H; Zúñiga-Pflücker, JC
Published Date
- July 2006
Published In
Volume / Issue
- 25 / 1
Start / End Page
- 105 - 116
PubMed ID
- 16814577
International Standard Serial Number (ISSN)
- 1074-7613
Digital Object Identifier (DOI)
- 10.1016/j.immuni.2006.05.010
Language
- eng
Conference Location
- United States