Stage-specific and differential notch dependency at the alphabeta and gammadelta T lineage bifurcation.

Published

Journal Article

Signals transduced by Notch receptors are indispensable for T cell specification and differentiation of alphabeta T lineage cells. However, the role of Notch signals during alphabeta versus gammadelta T lineage decision remains controversial. Here, we addressed this question by employing a clonal analysis of CD4(-)CD8(-) (DN) progenitor potential to position the divergence of alphabeta and gammadelta T cell lineages to the late DN2 to DN3 developmental stages. Accordingly, alphabeta and gammadelta precursor frequencies within these T cell progenitor subsets were determined, both in the presence and absence of Notch signaling through Delta-like 1. Notch signals were found to be critical for the DN to CD4(+)CD8(+) (DP) transition, irrespective of the identity (pTalphabeta or gammadelta) of the inducing T cell receptor complex, whereas gammadelta T cells developed from gammadeltaTCR-expressing T cell progenitors in the absence of further Notch ligand interaction. Collectively, our findings demonstrate a differential, stage-specific requirement for Notch receptor-ligand interactions in the differentiation of alphabeta and gammadelta T cells from T cell progenitors.

Full Text

Duke Authors

Cited Authors

  • Ciofani, M; Knowles, GC; Wiest, DL; von Boehmer, H; Zúñiga-Pflücker, JC

Published Date

  • July 2006

Published In

Volume / Issue

  • 25 / 1

Start / End Page

  • 105 - 116

PubMed ID

  • 16814577

Pubmed Central ID

  • 16814577

International Standard Serial Number (ISSN)

  • 1074-7613

Digital Object Identifier (DOI)

  • 10.1016/j.immuni.2006.05.010

Language

  • eng

Conference Location

  • United States