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RNA∶DNA hybrids initiate quasi-palindrome-associated mutations in highly transcribed yeast DNA.

Publication ,  Journal Article
Kim, N; Cho, J-E; Li, YC; Jinks-Robertson, S
Published in: PLoS Genet
November 2013

RNase H enzymes promote genetic stability by degrading aberrant RNA:DNA hybrids and by removing ribonucleotide monophosphates (rNMPs) that are present in duplex DNA. Here, we report that loss of RNase H2 in yeast is associated with mutations that extend identity between the arms of imperfect inverted repeats (quasi-palindromes or QPs), a mutation type generally attributed to a template switch during DNA synthesis. QP events were detected using frameshift-reversion assays and were only observed under conditions of high transcription. In striking contrast to transcription-associated short deletions that also are detected by these assays, QP events do not require Top1 activity. QP mutation rates are strongly affected by the direction of DNA replication and, in contrast to their elevation in the absence of RNase H2, are reduced when RNase H1 is additionally eliminated. Finally, transcription-associated QP events are limited by components of the nucleotide excision repair pathway and are promoted by translesion synthesis DNA polymerases. We suggest that QP mutations reflect either a transcription-associated perturbation of Okazaki-fragment processing, or the use of a nascent transcript to resume replication following a transcription-replication conflict.

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Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

November 2013

Volume

9

Issue

11

Start / End Page

e1003924

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Ribonucleotides
  • Ribonuclease H
  • RNA
  • Inverted Repeat Sequences
  • Frameshift Mutation
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA Repair
  • DNA
 

Citation

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Chicago
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Kim, N., Cho, J.-E., Li, Y. C., & Jinks-Robertson, S. (2013). RNA∶DNA hybrids initiate quasi-palindrome-associated mutations in highly transcribed yeast DNA. PLoS Genet, 9(11), e1003924. https://doi.org/10.1371/journal.pgen.1003924
Kim, Nayun, Jang-Eun Cho, Yue C. Li, and Sue Jinks-Robertson. “RNA∶DNA hybrids initiate quasi-palindrome-associated mutations in highly transcribed yeast DNA.PLoS Genet 9, no. 11 (November 2013): e1003924. https://doi.org/10.1371/journal.pgen.1003924.
Kim N, Cho J-E, Li YC, Jinks-Robertson S. RNA∶DNA hybrids initiate quasi-palindrome-associated mutations in highly transcribed yeast DNA. PLoS Genet. 2013 Nov;9(11):e1003924.
Kim, Nayun, et al. “RNA∶DNA hybrids initiate quasi-palindrome-associated mutations in highly transcribed yeast DNA.PLoS Genet, vol. 9, no. 11, Nov. 2013, p. e1003924. Pubmed, doi:10.1371/journal.pgen.1003924.
Kim N, Cho J-E, Li YC, Jinks-Robertson S. RNA∶DNA hybrids initiate quasi-palindrome-associated mutations in highly transcribed yeast DNA. PLoS Genet. 2013 Nov;9(11):e1003924.

Published In

PLoS Genet

DOI

EISSN

1553-7404

Publication Date

November 2013

Volume

9

Issue

11

Start / End Page

e1003924

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Ribonucleotides
  • Ribonuclease H
  • RNA
  • Inverted Repeat Sequences
  • Frameshift Mutation
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA Repair
  • DNA