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Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers.

Publication ,  Journal Article
Calin, GA; Sevignani, C; Dumitru, CD; Hyslop, T; Noch, E; Yendamuri, S; Shimizu, M; Rattan, S; Bullrich, F; Negrini, M; Croce, CM
Published in: Proc Natl Acad Sci U S A
March 2, 2004

A large number of tiny noncoding RNAs have been cloned and named microRNAs (miRs). Recently, we have reported that miR-15a and miR-16a, located at 13q14, are frequently deleted and/or down-regulated in patients with B cell chronic lymphocytic leukemia, a disorder characterized by increased survival. To further investigate the possible involvement of miRs in human cancers on a genome-wide basis, we have mapped 186 miRs and compared their location to the location of previous reported nonrandom genetic alterations. Here, we show that miR genes are frequently located at fragile sites, as well as in minimal regions of loss of heterozygosity, minimal regions of amplification (minimal amplicons), or common breakpoint regions. Overall, 98 of 186 (52.5%) of miR genes are in cancer-associated genomic regions or in fragile sites. Moreover, by Northern blotting, we have shown that several miRs located in deleted regions have low levels of expression in cancer samples. These data provide a catalog of miR genes that may have roles in cancer and argue that the full complement of miRs in a genome may be extensively involved in cancers.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 2, 2004

Volume

101

Issue

9

Start / End Page

2999 / 3004

Location

United States

Related Subject Headings

  • PubMed
  • Neoplasms
  • Multigene Family
  • MicroRNAs
  • Loss of Heterozygosity
  • Humans
  • Genome, Human
  • Genetic Markers
  • Genes, Homeobox
  • Databases, Nucleic Acid
 

Citation

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Calin, G. A., Sevignani, C., Dumitru, C. D., Hyslop, T., Noch, E., Yendamuri, S., … Croce, C. M. (2004). Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci U S A, 101(9), 2999–3004. https://doi.org/10.1073/pnas.0307323101
Calin, George Adrian, Cinzia Sevignani, Calin Dan Dumitru, Terry Hyslop, Evan Noch, Sai Yendamuri, Masayoshi Shimizu, et al. “Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers.Proc Natl Acad Sci U S A 101, no. 9 (March 2, 2004): 2999–3004. https://doi.org/10.1073/pnas.0307323101.
Calin GA, Sevignani C, Dumitru CD, Hyslop T, Noch E, Yendamuri S, et al. Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2999–3004.
Calin, George Adrian, et al. “Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers.Proc Natl Acad Sci U S A, vol. 101, no. 9, Mar. 2004, pp. 2999–3004. Pubmed, doi:10.1073/pnas.0307323101.
Calin GA, Sevignani C, Dumitru CD, Hyslop T, Noch E, Yendamuri S, Shimizu M, Rattan S, Bullrich F, Negrini M, Croce CM. Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc Natl Acad Sci U S A. 2004 Mar 2;101(9):2999–3004.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 2, 2004

Volume

101

Issue

9

Start / End Page

2999 / 3004

Location

United States

Related Subject Headings

  • PubMed
  • Neoplasms
  • Multigene Family
  • MicroRNAs
  • Loss of Heterozygosity
  • Humans
  • Genome, Human
  • Genetic Markers
  • Genes, Homeobox
  • Databases, Nucleic Acid