In vitro electrophoresis and in vivo electrophysiology of peripheral nerve using DC field stimulation.
BACKGROUND: Given the movement of molecules within tissue that occurs naturally by endogenous electric fields, we examined the possibility of using a low-voltage DC field to move charged substances in rodent peripheral nerve in vitro. NEW METHOD: Labeled sugar- and protein-based markers were applied to a rodent peroneal nerve and then a 5-10 V/cm field was used to move the molecules within the extra- and intraneural compartments. Physiological and anatomical nerve properties were also assessed using the same stimulation in vivo. RESULTS: We demonstrate in vitro that charged and labeled compounds are capable of moving in a DC field along a nerve, and that the same field applied in vivo changes the excitability of the nerve, but without damage. CONCLUSIONS: The results suggest that low-voltage electrophoresis could be used to move charged molecules, perhaps therapeutically, safely along peripheral nerves.
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- Rats
- Peroneal Nerve
- Neurology & Neurosurgery
- Mice, Transgenic
- Mice
- Electrophysiology
- Electrophoresis
- Electric Stimulation
- Biological Transport
- Animals
Citation
Published In
DOI
EISSN
Publication Date
Volume
Start / End Page
Location
Related Subject Headings
- Rats
- Peroneal Nerve
- Neurology & Neurosurgery
- Mice, Transgenic
- Mice
- Electrophysiology
- Electrophoresis
- Electric Stimulation
- Biological Transport
- Animals