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Whole-exome sequencing identifies rare and low-frequency coding variants associated with LDL cholesterol.

Publication ,  Journal Article
Lange, LA; Hu, Y; Zhang, H; Xue, C; Schmidt, EM; Tang, Z-Z; Bizon, C; Lange, EM; Smith, JD; Turner, EH; Jun, G; Kang, HM; Peloso, G; Auer, P ...
Published in: Am J Hum Genet
February 6, 2014

Elevated low-density lipoprotein cholesterol (LDL-C) is a treatable, heritable risk factor for cardiovascular disease. Genome-wide association studies (GWASs) have identified 157 variants associated with lipid levels but are not well suited to assess the impact of rare and low-frequency variants. To determine whether rare or low-frequency coding variants are associated with LDL-C, we exome sequenced 2,005 individuals, including 554 individuals selected for extreme LDL-C (>98(th) or <2(nd) percentile). Follow-up analyses included sequencing of 1,302 additional individuals and genotype-based analysis of 52,221 individuals. We observed significant evidence of association between LDL-C and the burden of rare or low-frequency variants in PNPLA5, encoding a phospholipase-domain-containing protein, and both known and previously unidentified variants in PCSK9, LDLR and APOB, three known lipid-related genes. The effect sizes for the burden of rare variants for each associated gene were substantially higher than those observed for individual SNPs identified from GWASs. We replicated the PNPLA5 signal in an independent large-scale sequencing study of 2,084 individuals. In conclusion, this large whole-exome-sequencing study for LDL-C identified a gene not known to be implicated in LDL-C and provides unique insight into the design and analysis of similar experiments.

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Published In

Am J Hum Genet

DOI

EISSN

1537-6605

Publication Date

February 6, 2014

Volume

94

Issue

2

Start / End Page

233 / 245

Location

United States

Related Subject Headings

  • Serine Endopeptidases
  • Sequence Analysis, DNA
  • Receptors, LDL
  • Proprotein Convertases
  • Proprotein Convertase 9
  • Polymorphism, Single Nucleotide
  • Phenotype
  • Middle Aged
  • Male
  • Lipase
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lange, L. A., Hu, Y., Zhang, H., Xue, C., Schmidt, E. M., Tang, Z.-Z., … NHLBI Grand Opportunity Exome Sequencing Project, . (2014). Whole-exome sequencing identifies rare and low-frequency coding variants associated with LDL cholesterol. Am J Hum Genet, 94(2), 233–245. https://doi.org/10.1016/j.ajhg.2014.01.010
Lange, Leslie A., Youna Hu, He Zhang, Chenyi Xue, Ellen M. Schmidt, Zheng-Zheng Tang, Chris Bizon, et al. “Whole-exome sequencing identifies rare and low-frequency coding variants associated with LDL cholesterol.Am J Hum Genet 94, no. 2 (February 6, 2014): 233–45. https://doi.org/10.1016/j.ajhg.2014.01.010.
Lange LA, Hu Y, Zhang H, Xue C, Schmidt EM, Tang Z-Z, et al. Whole-exome sequencing identifies rare and low-frequency coding variants associated with LDL cholesterol. Am J Hum Genet. 2014 Feb 6;94(2):233–45.
Lange, Leslie A., et al. “Whole-exome sequencing identifies rare and low-frequency coding variants associated with LDL cholesterol.Am J Hum Genet, vol. 94, no. 2, Feb. 2014, pp. 233–45. Pubmed, doi:10.1016/j.ajhg.2014.01.010.
Lange LA, Hu Y, Zhang H, Xue C, Schmidt EM, Tang Z-Z, Bizon C, Lange EM, Smith JD, Turner EH, Jun G, Kang HM, Peloso G, Auer P, Li K-P, Flannick J, Zhang J, Fuchsberger C, Gaulton K, Lindgren C, Locke A, Manning A, Sim X, Rivas MA, Holmen OL, Gottesman O, Lu Y, Ruderfer D, Stahl EA, Duan Q, Li Y, Durda P, Jiao S, Isaacs A, Hofman A, Bis JC, Correa A, Griswold ME, Jakobsdottir J, Smith AV, Schreiner PJ, Feitosa MF, Zhang Q, Huffman JE, Crosby J, Wassel CL, Do R, Franceschini N, Martin LW, Robinson JG, Assimes TL, Crosslin DR, Rosenthal EA, Tsai M, Rieder MJ, Farlow DN, Folsom AR, Lumley T, Fox ER, Carlson CS, Peters U, Jackson RD, van Duijn CM, Uitterlinden AG, Levy D, Rotter JI, Taylor HA, Gudnason V, Siscovick DS, Fornage M, Borecki IB, Hayward C, Rudan I, Chen YE, Bottinger EP, Loos RJF, Sætrom P, Hveem K, Boehnke M, Groop L, McCarthy M, Meitinger T, Ballantyne CM, Gabriel SB, O’Donnell CJ, Post WS, North KE, Reiner AP, Boerwinkle E, Psaty BM, Altshuler D, Kathiresan S, Lin D-Y, Jarvik GP, Cupples LA, Kooperberg C, Wilson JG, Nickerson DA, Abecasis GR, Rich SS, Tracy RP, Willer CJ, NHLBI Grand Opportunity Exome Sequencing Project. Whole-exome sequencing identifies rare and low-frequency coding variants associated with LDL cholesterol. Am J Hum Genet. 2014 Feb 6;94(2):233–245.
Journal cover image

Published In

Am J Hum Genet

DOI

EISSN

1537-6605

Publication Date

February 6, 2014

Volume

94

Issue

2

Start / End Page

233 / 245

Location

United States

Related Subject Headings

  • Serine Endopeptidases
  • Sequence Analysis, DNA
  • Receptors, LDL
  • Proprotein Convertases
  • Proprotein Convertase 9
  • Polymorphism, Single Nucleotide
  • Phenotype
  • Middle Aged
  • Male
  • Lipase