An antibody to the receptor for insulin-like growth factor I inhibits the growth of MCF-7 cells in tissue culture.
Alpha IR-3, a monoclonal antibody to the insulin-like growth factor I receptor which blocks insulin-like growth factor I binding and inhibits its activity, inhibits the binding of 125I-insulin-like growth factor I to MCF-7 cells (an estrogen dependent human breast carcinoma cell line) with an IC-50 of approximately 100 ng/ml. It also inhibits the growth of MCF-7 cells cultured in 5% calf serum with approximately the same IC-50. Inhibition of growth occurs both when cells are cultured in the presence and absence of estrogen and is more pronounced when cells are grown at a low density. These findings demonstrate a requirement for insulin-like growth factor I for optimal growth of MCF-7 cells and suggest that it is an autocrine growth factor in these cells.
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- Tumor Cells, Cultured
- Receptors, Somatomedin
- Receptor, Insulin
- Mice, Inbred BALB C
- Mice
- Insulin-Like Growth Factor I
- Humans
- Estradiol
- Cell Division
- Cell Count
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tumor Cells, Cultured
- Receptors, Somatomedin
- Receptor, Insulin
- Mice, Inbred BALB C
- Mice
- Insulin-Like Growth Factor I
- Humans
- Estradiol
- Cell Division
- Cell Count