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Identification of early interactions between Francisella and the host.

Publication ,  Journal Article
Roberts, LM; Tuladhar, S; Steele, SP; Riebe, KJ; Chen, C-J; Cumming, RI; Seay, S; Frothingham, R; Sempowski, GD; Kawula, TH; Frelinger, JA
Published in: Infect Immun
June 2014

The adaptive immune response to Francisella tularensis is dependent on the route of inoculation. Intradermal inoculation with the F. tularensis live vaccine strain (LVS) results in a robust Th1 response in the lungs, whereas intranasal inoculation produces fewer Th1 cells and instead many Th17 cells. Interestingly, bacterial loads in the lungs are similar early after inoculation by these two routes. We hypothesize that the adaptive immune response is influenced by local events in the lungs, such as the type of cells that are first infected with Francisella. Using fluorescence-activated cell sorting, we identified alveolar macrophages as the first cell type infected in the lungs of mice intranasally inoculated with F. novicida U112, LVS, or F. tularensis Schu S4. Following bacterial dissemination from the skin to the lung, interstitial macrophages or neutrophils are infected. Overall, we identified the early interactions between Francisella and the host following two different routes of inoculation.

Duke Scholars

Published In

Infect Immun

DOI

EISSN

1098-5522

Publication Date

June 2014

Volume

82

Issue

6

Start / End Page

2504 / 2510

Location

United States

Related Subject Headings

  • Tularemia
  • Pulmonary Alveoli
  • Neutrophils
  • Microbiology
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Lung
  • Host-Pathogen Interactions
  • Francisella tularensis
 

Citation

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Roberts, L. M., Tuladhar, S., Steele, S. P., Riebe, K. J., Chen, C.-J., Cumming, R. I., … Frelinger, J. A. (2014). Identification of early interactions between Francisella and the host. Infect Immun, 82(6), 2504–2510. https://doi.org/10.1128/IAI.01654-13
Roberts, Lydia M., Shraddha Tuladhar, Shaun P. Steele, Kristina J. Riebe, Ching-Ju Chen, R Ian Cumming, Sarah Seay, et al. “Identification of early interactions between Francisella and the host.Infect Immun 82, no. 6 (June 2014): 2504–10. https://doi.org/10.1128/IAI.01654-13.
Roberts LM, Tuladhar S, Steele SP, Riebe KJ, Chen C-J, Cumming RI, et al. Identification of early interactions between Francisella and the host. Infect Immun. 2014 Jun;82(6):2504–10.
Roberts, Lydia M., et al. “Identification of early interactions between Francisella and the host.Infect Immun, vol. 82, no. 6, June 2014, pp. 2504–10. Pubmed, doi:10.1128/IAI.01654-13.
Roberts LM, Tuladhar S, Steele SP, Riebe KJ, Chen C-J, Cumming RI, Seay S, Frothingham R, Sempowski GD, Kawula TH, Frelinger JA. Identification of early interactions between Francisella and the host. Infect Immun. 2014 Jun;82(6):2504–2510.

Published In

Infect Immun

DOI

EISSN

1098-5522

Publication Date

June 2014

Volume

82

Issue

6

Start / End Page

2504 / 2510

Location

United States

Related Subject Headings

  • Tularemia
  • Pulmonary Alveoli
  • Neutrophils
  • Microbiology
  • Mice, Inbred C57BL
  • Mice
  • Macrophages
  • Lung
  • Host-Pathogen Interactions
  • Francisella tularensis