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Transgenic overproduction of islet amyloid polypeptide (amylin) is not sufficient for islet amyloid formation.

Publication ,  Journal Article
Verchere, CB; D'Alessio, DA; Wang, S; Andrikopoulos, S; Kahn, SE
Published in: Horm Metab Res
June 1997

Islet amyloid polypeptide forms islet amyloid deposits in non-insulin-dependent diabetes mellitus. We have generated transgenic mice which express human islet amyloid polypeptide in their pancreatic beta cells yet do not develop islet amyloid deposits despite producing levels of the amyloidogenic human peptide 2 - 3 fold higher than the native (mouse) peptide. To determine whether marked overproduction of islet amyloid polypeptide is a potential cause of islet amyloid formation, we increased expression of this transgene by producing homozygous transgenic animals and by making heterozygous mice experimentally insulin resistant with nicotinic acid. Pancreatic content of islet amyloid polypeptide-like immunoreactivity in homozygous and nicotinic acid-treated mice was 2-fold (25 +/- 7 fmol/microg; n = 6) and 3.5-fold (47 +/- 20 fmol/microg; n = 3) higher, respectively, than that of untreated heterozygous animals (13+/-2 fmol/microg; n = 11; both p < 0.05). Despite this marked increase in production of islet amyloid polypeptide, neither group of mice developed gross islet amyloid deposits even after 16 months of age. We conclude that overproduction of islet amyloid polypeptide, even as produced by extreme insulin resistance, is not in itself sufficient for islet amyloid formation.

Duke Scholars

Published In

Horm Metab Res

DOI

ISSN

0018-5043

Publication Date

June 1997

Volume

29

Issue

6

Start / End Page

311 / 316

Location

Germany

Related Subject Headings

  • Pancreas
  • Mice, Transgenic
  • Mice
  • Islets of Langerhans
  • Islet Amyloid Polypeptide
  • Humans
  • Endocrinology & Metabolism
  • Animals
  • Amyloid
  • 3202 Clinical sciences
 

Citation

APA
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MLA
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Verchere, C. B., D’Alessio, D. A., Wang, S., Andrikopoulos, S., & Kahn, S. E. (1997). Transgenic overproduction of islet amyloid polypeptide (amylin) is not sufficient for islet amyloid formation. Horm Metab Res, 29(6), 311–316. https://doi.org/10.1055/s-2007-979042
Verchere, C. B., D. A. D’Alessio, S. Wang, S. Andrikopoulos, and S. E. Kahn. “Transgenic overproduction of islet amyloid polypeptide (amylin) is not sufficient for islet amyloid formation.Horm Metab Res 29, no. 6 (June 1997): 311–16. https://doi.org/10.1055/s-2007-979042.
Verchere CB, D’Alessio DA, Wang S, Andrikopoulos S, Kahn SE. Transgenic overproduction of islet amyloid polypeptide (amylin) is not sufficient for islet amyloid formation. Horm Metab Res. 1997 Jun;29(6):311–6.
Verchere, C. B., et al. “Transgenic overproduction of islet amyloid polypeptide (amylin) is not sufficient for islet amyloid formation.Horm Metab Res, vol. 29, no. 6, June 1997, pp. 311–16. Pubmed, doi:10.1055/s-2007-979042.
Verchere CB, D’Alessio DA, Wang S, Andrikopoulos S, Kahn SE. Transgenic overproduction of islet amyloid polypeptide (amylin) is not sufficient for islet amyloid formation. Horm Metab Res. 1997 Jun;29(6):311–316.
Journal cover image

Published In

Horm Metab Res

DOI

ISSN

0018-5043

Publication Date

June 1997

Volume

29

Issue

6

Start / End Page

311 / 316

Location

Germany

Related Subject Headings

  • Pancreas
  • Mice, Transgenic
  • Mice
  • Islets of Langerhans
  • Islet Amyloid Polypeptide
  • Humans
  • Endocrinology & Metabolism
  • Animals
  • Amyloid
  • 3202 Clinical sciences