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Effect of somatostatin-28 on dynamics of insulin secretion in perfused rat pancreas.

Publication ,  Journal Article
Ensinck, JW; Laschansky, EC; Vogel, RE; D'Alessio, DA
Published in: Diabetes
September 1991

Somatostatin-28 (S-28), originating in gastrointestinal cells, is secreted into the circulatory system and rises in human plasma after ingestion of a mixed meal. Pancreatic beta-cells contain specific, high-affinity receptors for S-28, and it is plausible that this peptide is a physiological modulator of insulin secretion. To evaluate the effects of physiological concentrations of S-28 on glucose-mediated insulin secretion, we used the perfused in situ rat pancreas under two conditions: 1) "square-wave" glucose infusion from 2.8 to 11.1 mM and 2) ramping of glucose at 0.28 mM/min throughout 45 min. S-28 concentrations of 16, 32, and 80 pM were separately coinfused for 40 min in the first condition and at 16 pM in the second condition. During square-wave glucose infusion, biphasic insulin secretion was elicited with marked attenuation of both phases during coinfusion with the two higher concentrations of S-28. At 16 pM S-28, which approximates postprandial At 16 pM S-28, which approximates postprandial concentrations, only first-phase secretion was suppressed. During ramping of glucose, insulin was released gradually and, in the presence of 16 pM S-28, was shifted to the right, indicating an increase in threshold glucose levels for insulin secretion. We concluded that S-28, at levels achieved postprandially, modulates the release of insulin by altering the threshold of sensitivity to glucose.

Duke Scholars

Published In

Diabetes

DOI

ISSN

0012-1797

Publication Date

September 1991

Volume

40

Issue

9

Start / End Page

1163 / 1169

Location

United States

Related Subject Headings

  • Time Factors
  • Somatostatin-28
  • Somatostatin
  • Reference Values
  • Rats, Inbred Strains
  • Rats
  • Protein Precursors
  • Perfusion
  • Middle Aged
  • Male
 

Citation

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Chicago
ICMJE
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Ensinck, J. W., Laschansky, E. C., Vogel, R. E., & D’Alessio, D. A. (1991). Effect of somatostatin-28 on dynamics of insulin secretion in perfused rat pancreas. Diabetes, 40(9), 1163–1169. https://doi.org/10.2337/diab.40.9.1163
Ensinck, J. W., E. C. Laschansky, R. E. Vogel, and D. A. D’Alessio. “Effect of somatostatin-28 on dynamics of insulin secretion in perfused rat pancreas.Diabetes 40, no. 9 (September 1991): 1163–69. https://doi.org/10.2337/diab.40.9.1163.
Ensinck JW, Laschansky EC, Vogel RE, D’Alessio DA. Effect of somatostatin-28 on dynamics of insulin secretion in perfused rat pancreas. Diabetes. 1991 Sep;40(9):1163–9.
Ensinck, J. W., et al. “Effect of somatostatin-28 on dynamics of insulin secretion in perfused rat pancreas.Diabetes, vol. 40, no. 9, Sept. 1991, pp. 1163–69. Pubmed, doi:10.2337/diab.40.9.1163.
Ensinck JW, Laschansky EC, Vogel RE, D’Alessio DA. Effect of somatostatin-28 on dynamics of insulin secretion in perfused rat pancreas. Diabetes. 1991 Sep;40(9):1163–1169.

Published In

Diabetes

DOI

ISSN

0012-1797

Publication Date

September 1991

Volume

40

Issue

9

Start / End Page

1163 / 1169

Location

United States

Related Subject Headings

  • Time Factors
  • Somatostatin-28
  • Somatostatin
  • Reference Values
  • Rats, Inbred Strains
  • Rats
  • Protein Precursors
  • Perfusion
  • Middle Aged
  • Male