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Cell senescence reduced the mechanotransduction sensitivity of porcine angular aqueous plexus cells to elevation of pressure.

Publication ,  Journal Article
Lei, Y; Stamer, WD; Wu, J; Sun, X
Published in: Invest Ophthalmol Vis Sci
April 11, 2014

PURPOSE: To investigate the effect of pressure/flow on the barrier function and protein expression of normal and senescent porcine aqueous humor plexus (AAP) cells, which are the porcine equivalent of human Schlemm's canal endothelial cells. METHODS: AAP cells were grown for 2 weeks in physiological (5% O2) or hyperoxic conditions (40% O2) to model cell senescence. Control and senescent AAP cells were subjected to control and elevated hydrostatic pressure gradient of 10 mm Hg for 72 hours. Hydraulic conductivity (HC) and transendothelial electric resistance (TEER) were measured. The expressions of senescence-associated β-galactosidase and DNA damage marker 8-hydroxy-2'-deoxyguanosine (8-OHdG) were monitored, and the protein expression profile was analyzed by Western blot. RESULTS: After 14 days of hyperoxia, AAP cells stained positive for 8-OHdG and β-galactosidase. Pressure elevation/flow resulted in significant increase of HC in control cells (from 1.37 ± 0.12 to 1.64 ± 0.18 μL/mm Hg/min/cm(2), P < 0.05), but not in senescent cells (1.15 ± 0.17 and 1.08 ± 0.10 μL μL/mm Hg/min/cm(2)). TEER changes were consistent with the HC results. Western blot analysis showed that the expression level of myosin light chain, claudin-5, and VE-cadherin significantly reduced under pressure elevation in control cells but not in senescent cells. CONCLUSIONS: AAP cells are mechano-sensitive; however, cell senescence rendered the cells less responsive to mechanical stimulus, which may have pathological consequences.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

April 11, 2014

Volume

55

Issue

4

Start / End Page

2324 / 2328

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Swine
  • Oxidative Stress
  • Ophthalmology & Optometry
  • Mechanotransduction, Cellular
  • Intraocular Pressure
  • Glaucoma
  • Follow-Up Studies
  • Disease Models, Animal
  • Cellular Senescence
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lei, Y., Stamer, W. D., Wu, J., & Sun, X. (2014). Cell senescence reduced the mechanotransduction sensitivity of porcine angular aqueous plexus cells to elevation of pressure. Invest Ophthalmol Vis Sci, 55(4), 2324–2328. https://doi.org/10.1167/iovs.13-13317
Lei, Yuan, William D. Stamer, Jihong Wu, and Xinghuai Sun. “Cell senescence reduced the mechanotransduction sensitivity of porcine angular aqueous plexus cells to elevation of pressure.Invest Ophthalmol Vis Sci 55, no. 4 (April 11, 2014): 2324–28. https://doi.org/10.1167/iovs.13-13317.
Lei Y, Stamer WD, Wu J, Sun X. Cell senescence reduced the mechanotransduction sensitivity of porcine angular aqueous plexus cells to elevation of pressure. Invest Ophthalmol Vis Sci. 2014 Apr 11;55(4):2324–8.
Lei, Yuan, et al. “Cell senescence reduced the mechanotransduction sensitivity of porcine angular aqueous plexus cells to elevation of pressure.Invest Ophthalmol Vis Sci, vol. 55, no. 4, Apr. 2014, pp. 2324–28. Pubmed, doi:10.1167/iovs.13-13317.
Lei Y, Stamer WD, Wu J, Sun X. Cell senescence reduced the mechanotransduction sensitivity of porcine angular aqueous plexus cells to elevation of pressure. Invest Ophthalmol Vis Sci. 2014 Apr 11;55(4):2324–2328.

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

April 11, 2014

Volume

55

Issue

4

Start / End Page

2324 / 2328

Location

United States

Related Subject Headings

  • Trabecular Meshwork
  • Swine
  • Oxidative Stress
  • Ophthalmology & Optometry
  • Mechanotransduction, Cellular
  • Intraocular Pressure
  • Glaucoma
  • Follow-Up Studies
  • Disease Models, Animal
  • Cellular Senescence