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Delayed transition to new cell fates during cellular reprogramming.

Publication ,  Journal Article
Cheng, X; Lyons, DC; Socolar, JES; McClay, DR
Published in: Developmental biology
July 2014

In many embryos specification toward one cell fate can be diverted to a different cell fate through a reprogramming process. Understanding how that process works will reveal insights into the developmental regulatory logic that emerged from evolution. In the sea urchin embryo, cells at gastrulation were found to reprogram and replace missing cell types after surgical dissections of the embryo. Non-skeletogenic mesoderm (NSM) cells reprogrammed to replace missing skeletogenic mesoderm cells and animal caps reprogrammed to replace all endomesoderm. In both cases evidence of reprogramming onset was first observed at the early gastrula stage, even if the cells to be replaced were removed earlier in development. Once started however, the reprogramming occurred with compressed gene expression dynamics. The NSM did not require early contact with the skeletogenic cells to reprogram, but the animal cap cells gained the ability to reprogram early in gastrulation only after extended contact with the vegetal halves prior to that time. If the entire vegetal half was removed at early gastrula, the animal caps reprogrammed and replaced the vegetal half endomesoderm. If the animal caps carried morpholinos to either hox11/13b or foxA (endomesoderm specification genes), the isolated animal caps failed to reprogram. Together these data reveal that the emergence of a reprogramming capability occurs at early gastrulation in the sea urchin embryo and requires activation of early specification components of the target tissues.

Duke Scholars

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Published In

Developmental biology

DOI

EISSN

1095-564X

ISSN

0012-1606

Publication Date

July 2014

Volume

391

Issue

2

Start / End Page

147 / 157

Related Subject Headings

  • Signal Transduction
  • Mesoderm
  • Lytechinus
  • Gene Expression Regulation, Developmental
  • Gastrulation
  • Gastrula
  • Embryo, Nonmammalian
  • Developmental Biology
  • Cellular Reprogramming
  • Cell Differentiation
 

Citation

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Cheng, X., Lyons, D. C., Socolar, J. E. S., & McClay, D. R. (2014). Delayed transition to new cell fates during cellular reprogramming. Developmental Biology, 391(2), 147–157. https://doi.org/10.1016/j.ydbio.2014.04.015
Cheng, Xianrui, Deirdre C. Lyons, Joshua E. S. Socolar, and David R. McClay. “Delayed transition to new cell fates during cellular reprogramming.Developmental Biology 391, no. 2 (July 2014): 147–57. https://doi.org/10.1016/j.ydbio.2014.04.015.
Cheng X, Lyons DC, Socolar JES, McClay DR. Delayed transition to new cell fates during cellular reprogramming. Developmental biology. 2014 Jul;391(2):147–57.
Cheng, Xianrui, et al. “Delayed transition to new cell fates during cellular reprogramming.Developmental Biology, vol. 391, no. 2, July 2014, pp. 147–57. Epmc, doi:10.1016/j.ydbio.2014.04.015.
Cheng X, Lyons DC, Socolar JES, McClay DR. Delayed transition to new cell fates during cellular reprogramming. Developmental biology. 2014 Jul;391(2):147–157.
Journal cover image

Published In

Developmental biology

DOI

EISSN

1095-564X

ISSN

0012-1606

Publication Date

July 2014

Volume

391

Issue

2

Start / End Page

147 / 157

Related Subject Headings

  • Signal Transduction
  • Mesoderm
  • Lytechinus
  • Gene Expression Regulation, Developmental
  • Gastrulation
  • Gastrula
  • Embryo, Nonmammalian
  • Developmental Biology
  • Cellular Reprogramming
  • Cell Differentiation