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In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics.

Publication ,  Journal Article
Lacroix, B; Bourdages, KG; Dorn, JF; Ihara, S; Sherwood, DR; Maddox, PS; Maddox, AS
Published in: Developmental cell
April 2014

Microtubules (MTs) are cytoskeletal polymers that undergo dynamic instability, the stochastic transition between growth and shrinkage phases. MT dynamics are required for diverse cellular processes and, while intrinsic to tubulin, are highly regulated. However, little is known about how MT dynamics facilitate or are regulated by tissue biogenesis and differentiation. We imaged MT dynamics in a smooth muscle-like lineage in intact developing Caenorhabditis elegans. All aspects of MT dynamics change significantly as stem-like precursors exit mitosis and, secondarily, as they differentiate. We found that suppression, but not enhancement, of dynamics perturbs differentiated muscle function in vivo. Distinct ensembles of MT-associated proteins are specifically required for tissue biogenesis versus tissue function. A CLASP family MT stabilizer and the depolymerizing kinesin MCAK are differentially required for MT dynamics in the precursor or differentiated cells, respectively. All of these multidimensional phenotypic comparisons were facilitated by a data display method called the diamond graph.

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Published In

Developmental cell

DOI

EISSN

1878-1551

ISSN

1534-5807

Publication Date

April 2014

Volume

29

Issue

2

Start / End Page

203 / 216

Related Subject Headings

  • Tubulin
  • Spindle Apparatus
  • Reproduction
  • Ovum
  • Mitosis
  • Microtubules
  • Microtubule-Associated Proteins
  • Kinesins
  • Developmental Biology
  • Cell Lineage
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lacroix, B., Bourdages, K. G., Dorn, J. F., Ihara, S., Sherwood, D. R., Maddox, P. S., & Maddox, A. S. (2014). In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics. Developmental Cell, 29(2), 203–216. https://doi.org/10.1016/j.devcel.2014.03.007
Lacroix, Benjamin, Karine G. Bourdages, Jonas F. Dorn, Shinji Ihara, David R. Sherwood, Paul S. Maddox, and Amy S. Maddox. “In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics.Developmental Cell 29, no. 2 (April 2014): 203–16. https://doi.org/10.1016/j.devcel.2014.03.007.
Lacroix B, Bourdages KG, Dorn JF, Ihara S, Sherwood DR, Maddox PS, et al. In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics. Developmental cell. 2014 Apr;29(2):203–16.
Lacroix, Benjamin, et al. “In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics.Developmental Cell, vol. 29, no. 2, Apr. 2014, pp. 203–16. Epmc, doi:10.1016/j.devcel.2014.03.007.
Lacroix B, Bourdages KG, Dorn JF, Ihara S, Sherwood DR, Maddox PS, Maddox AS. In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics. Developmental cell. 2014 Apr;29(2):203–216.
Journal cover image

Published In

Developmental cell

DOI

EISSN

1878-1551

ISSN

1534-5807

Publication Date

April 2014

Volume

29

Issue

2

Start / End Page

203 / 216

Related Subject Headings

  • Tubulin
  • Spindle Apparatus
  • Reproduction
  • Ovum
  • Mitosis
  • Microtubules
  • Microtubule-Associated Proteins
  • Kinesins
  • Developmental Biology
  • Cell Lineage