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Establishment of an index with increased sensitivity for assessing murine arthritis.

Publication ,  Journal Article
Sampson, ER; Beck, CA; Ketz, J; Canary, KL; Hilton, MJ; Awad, H; Schwarz, EM; Chen, D; O'Keefe, RJ; Rosier, RN; Zuscik, MJ
Published in: J Orthop Res
August 2011

The goals of our study were to establish quantitative outcomes for assessing murine knee arthritis and develop an Arthritis Index that incorporates multiple outcomes into a single calculation that provides enhanced sensitivity. Using an accepted model of meniscal/ligamentous injury (MLI)-induced osteoarthritis (OA), we assessed mouse knee arthritis using several approaches. Histology-based methods were performed to visualize joint tissues including articular cartilage and subchondral bone. Accepted histologic scoring methods and histomorphometry were performed to grade cartilage degeneration and determine articular cartilage area, respectively. MicroCT was used to visualize and quantify the bony structures of the joint including osteophytes and joint bone volume. A statistical algorithm was then developed that combined histologic scores and cartilage areas into a single Arthritis Index. MLI induced progressive, OA-like articular cartilage degeneration characterized by increasing (worsening) histologic score and decreasing cartilage area. MicroCT revealed osteophytes and increased joint bone volume between the femoral and tibial physes following MLI. Lastly, an Arthritis Index calculation was established, which incorporated histologic scoring and cartilage area. The Arthritis Index provided enhanced quantitative sensitivity in assessing the level of joint degeneration compared to either histologic scoring or cartilage area determination alone; when using the Index, between 29% and 43% fewer samples are needed to establish statistical significance in studies of murine arthritis. Arthritis in the mouse knee can be quantitatively assessed by histologic scoring, measuring cartilage area, and determining joint bone volume. Enhanced sensitivity can be achieved by performing the Arthritis Index calculation, a novel method for quantitatively assessing mouse knee arthritis.

Duke Scholars

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

August 2011

Volume

29

Issue

8

Start / End Page

1145 / 1151

Location

United States

Related Subject Headings

  • Severity of Illness Index
  • Osteoarthritis, Knee
  • Orthopedics
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Hindlimb
  • Disease Models, Animal
  • Cartilage, Articular
  • Animals
 

Citation

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Sampson, E. R., Beck, C. A., Ketz, J., Canary, K. L., Hilton, M. J., Awad, H., … Zuscik, M. J. (2011). Establishment of an index with increased sensitivity for assessing murine arthritis. J Orthop Res, 29(8), 1145–1151. https://doi.org/10.1002/jor.21368
Sampson, Erik R., Christopher A. Beck, John Ketz, Krista L. Canary, Matthew J. Hilton, Hani Awad, Edward M. Schwarz, et al. “Establishment of an index with increased sensitivity for assessing murine arthritis.J Orthop Res 29, no. 8 (August 2011): 1145–51. https://doi.org/10.1002/jor.21368.
Sampson ER, Beck CA, Ketz J, Canary KL, Hilton MJ, Awad H, et al. Establishment of an index with increased sensitivity for assessing murine arthritis. J Orthop Res. 2011 Aug;29(8):1145–51.
Sampson, Erik R., et al. “Establishment of an index with increased sensitivity for assessing murine arthritis.J Orthop Res, vol. 29, no. 8, Aug. 2011, pp. 1145–51. Pubmed, doi:10.1002/jor.21368.
Sampson ER, Beck CA, Ketz J, Canary KL, Hilton MJ, Awad H, Schwarz EM, Chen D, O’Keefe RJ, Rosier RN, Zuscik MJ. Establishment of an index with increased sensitivity for assessing murine arthritis. J Orthop Res. 2011 Aug;29(8):1145–1151.
Journal cover image

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

August 2011

Volume

29

Issue

8

Start / End Page

1145 / 1151

Location

United States

Related Subject Headings

  • Severity of Illness Index
  • Osteoarthritis, Knee
  • Orthopedics
  • Mice, Inbred C57BL
  • Mice
  • Male
  • Hindlimb
  • Disease Models, Animal
  • Cartilage, Articular
  • Animals