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Chronic axial compression of the mouse tail segment induces MRI bone marrow edema changes that correlate with increased marrow vasculature and cellularity.

Publication ,  Journal Article
Papuga, MO; Proulx, ST; Kwok, E; You, Z; Rubery, PT; Dougherty, PE; Hilton, MJ; Awad, HA; Schwarz, EM
Published in: J Orthop Res
September 2010

Magnetic resonance imaging (MRI) of bone marrow edema (BME) has been found to be helpful in the diagnosis of back pain attributed to degenerative disk disease (DDD) and spondyloarthropathy (SA), but its interpretation is limited by a lack of knowledge of its nature and natural history. We assessed effects of compressive forces to mouse tail segments of WT and TNF-Tg mice with SA, via contrast enhanced-MRI and histology. Normalized marrow contrast enhancement (NMCE) of uninstrumented WT vertebrae significantly decrease, threefold (p < 0.01) from 8 to 12 weeks of age, while the NMCE of TNF-Tg vertebrae remained elevated. Compressive loading (6x body weight) increased NMCE twofold (p < 0.02) within 2 weeks in WT tails, which was equal to 6x loaded TNF-Tg tails within 4 weeks. Histology confirmed degenerative changes and that load-induced NMCE corresponded to increased vascular sinus tissue (35 +/- 3% vs. 19 +/- 3%; p < 0.01) and cellularity (4,235 +/- 886 vs.1,468 +/- 320 cells/mm(2); p < 0.01) for the loaded versus unloaded WT, respectively. However, micro-computed tomography (CT) analyses failed to detect significant load-induced changes to bone. While the bone marrow of loaded WT and TNF-Tg vertebrae were similar, histology demonstrated mild cellular infiltrate and increased osteoclastic resorption in the WT tails versus severe inflammatory-erosive arthritis in TNF-Tg joints. Significant (p < 0.05) decreases in cortical and trabecular bone volume in uninstrumented TNF-Tg versus WT vertebrae were confirmed by micro-CT. Thus, chronic load-induced DDD causes BME signals in vertebrae similar to those observed from SA, and both DDD and SA signals correlate with a conversion from yellow to red marrow, with increased vascularity.

Duke Scholars

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

September 2010

Volume

28

Issue

9

Start / End Page

1220 / 1228

Location

United States

Related Subject Headings

  • X-Ray Microtomography
  • Weight-Bearing
  • Tail
  • Spondylarthropathies
  • Spine
  • Osteoclasts
  • Orthopedics
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
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Papuga, M. O., Proulx, S. T., Kwok, E., You, Z., Rubery, P. T., Dougherty, P. E., … Schwarz, E. M. (2010). Chronic axial compression of the mouse tail segment induces MRI bone marrow edema changes that correlate with increased marrow vasculature and cellularity. J Orthop Res, 28(9), 1220–1228. https://doi.org/10.1002/jor.21103
Papuga, M Owen, Steven T. Proulx, Edmund Kwok, Zhigang You, Paul T. Rubery, Paul E. Dougherty, Matthew J. Hilton, Hani A. Awad, and Edward M. Schwarz. “Chronic axial compression of the mouse tail segment induces MRI bone marrow edema changes that correlate with increased marrow vasculature and cellularity.J Orthop Res 28, no. 9 (September 2010): 1220–28. https://doi.org/10.1002/jor.21103.
Papuga MO, Proulx ST, Kwok E, You Z, Rubery PT, Dougherty PE, et al. Chronic axial compression of the mouse tail segment induces MRI bone marrow edema changes that correlate with increased marrow vasculature and cellularity. J Orthop Res. 2010 Sep;28(9):1220–8.
Papuga, M. Owen, et al. “Chronic axial compression of the mouse tail segment induces MRI bone marrow edema changes that correlate with increased marrow vasculature and cellularity.J Orthop Res, vol. 28, no. 9, Sept. 2010, pp. 1220–28. Pubmed, doi:10.1002/jor.21103.
Papuga MO, Proulx ST, Kwok E, You Z, Rubery PT, Dougherty PE, Hilton MJ, Awad HA, Schwarz EM. Chronic axial compression of the mouse tail segment induces MRI bone marrow edema changes that correlate with increased marrow vasculature and cellularity. J Orthop Res. 2010 Sep;28(9):1220–1228.
Journal cover image

Published In

J Orthop Res

DOI

EISSN

1554-527X

Publication Date

September 2010

Volume

28

Issue

9

Start / End Page

1220 / 1228

Location

United States

Related Subject Headings

  • X-Ray Microtomography
  • Weight-Bearing
  • Tail
  • Spondylarthropathies
  • Spine
  • Osteoclasts
  • Orthopedics
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Mice