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The kinesin-13 KLP10A motor regulates oocyte spindle length and affects EB1 binding without altering microtubule growth rates.

Publication ,  Journal Article
Do, KK; Hoàng, KL; Endow, SA
Published in: Biol Open
June 6, 2014

Kinesin-13 motors are unusual in that they do not walk along microtubules, but instead diffuse to the ends, where they remove tubulin dimers, regulating microtubule dynamics. Here we show that Drosophila kinesin-13 klp10A regulates oocyte meiosis I spindle length and is haplo-insufficient - KLP10A, reduced by RNAi or a loss-of-function P element insertion mutant, results in elongated and mispositioned oocyte spindles, and abnormal cortical microtubule asters and aggregates. KLP10A knockdown by RNAi does not significantly affect microtubule growth rates in oocyte spindles, but, unexpectedly, EB1 binding and unbinding are slowed, suggesting a previously unobserved role for kinesin-13 in mediating EB1 binding interactions with microtubules. Kinesin-13 may regulate spindle length both by disassembling subunits from microtubule ends and facilitating EB1 binding to plus ends. We also observe an increased number of paused microtubules in klp10A RNAi knockdown spindles, consistent with a reduced frequency of microtubule catastrophes. Overall, our findings indicate that reduced kinesin-13 decreases microtubule disassembly rates and affects EB1 interactions with microtubules, rather than altering microtubule growth rates, causing spindles to elongate and abnormal cortical microtubule asters and aggregates to form.

Duke Scholars

Published In

Biol Open

DOI

ISSN

2046-6390

Publication Date

June 6, 2014

Volume

3

Issue

7

Start / End Page

561 / 570

Location

England

Related Subject Headings

  • 41 Environmental sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 0699 Other Biological Sciences
 

Citation

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Do, K. K., Hoàng, K. L., & Endow, S. A. (2014). The kinesin-13 KLP10A motor regulates oocyte spindle length and affects EB1 binding without altering microtubule growth rates. Biol Open, 3(7), 561–570. https://doi.org/10.1242/bio.20148276
Do, Kevin K., Kim Liên Hoàng, and Sharyn A. Endow. “The kinesin-13 KLP10A motor regulates oocyte spindle length and affects EB1 binding without altering microtubule growth rates.Biol Open 3, no. 7 (June 6, 2014): 561–70. https://doi.org/10.1242/bio.20148276.
Do, Kevin K., et al. “The kinesin-13 KLP10A motor regulates oocyte spindle length and affects EB1 binding without altering microtubule growth rates.Biol Open, vol. 3, no. 7, June 2014, pp. 561–70. Pubmed, doi:10.1242/bio.20148276.
Journal cover image

Published In

Biol Open

DOI

ISSN

2046-6390

Publication Date

June 6, 2014

Volume

3

Issue

7

Start / End Page

561 / 570

Location

England

Related Subject Headings

  • 41 Environmental sciences
  • 32 Biomedical and clinical sciences
  • 31 Biological sciences
  • 0699 Other Biological Sciences