Skip to main content
Journal cover image

Prognostic significance of differential expression of angiogenic genes in women with high-grade serous ovarian carcinoma.

Publication ,  Journal Article
Siamakpour-Reihani, S; Owzar, K; Jiang, C; Turner, T; Deng, Y; Bean, SM; Horton, JK; Berchuck, A; Marks, JR; Dewhirst, MW; Alvarez Secord, A
Published in: Gynecol Oncol
October 2015

OBJECTIVES: To identify angiogenic biomarkers associated with tumor angiogenesis and clinical outcome in high-grade serous ovarian cancer (HGSC). METHODS: 51 HGSC samples were analyzed using Affymetrix HG-U133A microarray. Microvessel density (MVD) counts were determined using CD31 and CD105. Associations between mRNA expression levels and overall survival were assessed using rank score statistic. Effect size was estimated as a hazard ratio (HR) under a proportional hazard model. The Storey q-value method was used to account for multiple testing within the false-discovery rate (FDR) framework. Publicly available databases including TCGA and GSE were used for external confirmation. RESULTS: Thirty-one angiogenic-related genes were significantly associated with survival (q≤0.05). Of these 31 genes, 4 were also associated with outcome in the TCGA data: AKT1 (q=0.02; TCGA p=0.01, HR=0.8), CD44 (q=0.003; TCGA p=0.05, HR=0.9), EPHB2 (q=0.01; TCGA p=0.05, HR=1.2), and ERBB2 (q=0.02; TCGA p=0.05, HR=1.2). While 5 were associated with outcome in the GSE database: FLT1 (q=0.03; GSE26712 p=0.01, HR=3.1); PF4 (q=0.02; GSE26712 p=0.01, HR=3.0); NRP1 (q=0.02; GSE26712 p<0.04, HR>1.4); COL4A3 (q=0.04; GSE26712 p=0.03, HR=1.3); and ANGPTL3 (q=0.02; GSE14764 p=0.02, HR=1.5). High AKT1 and CD44 were associated with longer survival. In contrast, high expression of EPHB2, ERBB2, FLT1; PF4, NRP1, COL4A3, and ANGPTL3 were associated with shorter survival. CD105-MVD and CD31-MVD were not significantly associated with angiogenic gene expression. CONCLUSIONS: Thirty-one angiogenic-related genes were associated with survival in advanced HGSC and nine of these genes were confirmed in independent publicly available databases.

Duke Scholars

Published In

Gynecol Oncol

DOI

EISSN

1095-6859

Publication Date

October 2015

Volume

139

Issue

1

Start / End Page

23 / 29

Location

United States

Related Subject Headings

  • Survival Rate
  • Receptors, Cell Surface
  • Proportional Hazards Models
  • Prognosis
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Ovarian Neoplasms
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neovascularization, Pathologic
  • Neoplasm Grading
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Siamakpour-Reihani, S., Owzar, K., Jiang, C., Turner, T., Deng, Y., Bean, S. M., … Alvarez Secord, A. (2015). Prognostic significance of differential expression of angiogenic genes in women with high-grade serous ovarian carcinoma. Gynecol Oncol, 139(1), 23–29. https://doi.org/10.1016/j.ygyno.2015.08.001
Siamakpour-Reihani, Sharareh, Kouros Owzar, Chen Jiang, Taylor Turner, Yiwen Deng, Sarah M. Bean, Janet K. Horton, et al. “Prognostic significance of differential expression of angiogenic genes in women with high-grade serous ovarian carcinoma.Gynecol Oncol 139, no. 1 (October 2015): 23–29. https://doi.org/10.1016/j.ygyno.2015.08.001.
Siamakpour-Reihani S, Owzar K, Jiang C, Turner T, Deng Y, Bean SM, et al. Prognostic significance of differential expression of angiogenic genes in women with high-grade serous ovarian carcinoma. Gynecol Oncol. 2015 Oct;139(1):23–9.
Siamakpour-Reihani, Sharareh, et al. “Prognostic significance of differential expression of angiogenic genes in women with high-grade serous ovarian carcinoma.Gynecol Oncol, vol. 139, no. 1, Oct. 2015, pp. 23–29. Pubmed, doi:10.1016/j.ygyno.2015.08.001.
Siamakpour-Reihani S, Owzar K, Jiang C, Turner T, Deng Y, Bean SM, Horton JK, Berchuck A, Marks JR, Dewhirst MW, Alvarez Secord A. Prognostic significance of differential expression of angiogenic genes in women with high-grade serous ovarian carcinoma. Gynecol Oncol. 2015 Oct;139(1):23–29.
Journal cover image

Published In

Gynecol Oncol

DOI

EISSN

1095-6859

Publication Date

October 2015

Volume

139

Issue

1

Start / End Page

23 / 29

Location

United States

Related Subject Headings

  • Survival Rate
  • Receptors, Cell Surface
  • Proportional Hazards Models
  • Prognosis
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Ovarian Neoplasms
  • Oncology & Carcinogenesis
  • Oligonucleotide Array Sequence Analysis
  • Neovascularization, Pathologic
  • Neoplasm Grading