Skip to main content

Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients.

Publication ,  Journal Article
Mikita, K; Thakur, K; Anstey, NM; Piera, KA; Pardo, CA; Weinberg, JB; Mukemba, J; Florence, S; Mwaikambo, ED; Granger, DL; Sullivan, DJ
Published in: Am J Trop Med Hyg
September 2014

A cerebrospinal fluid (CSF) biomarker for cerebral malaria (CM) has not been validated. We examined the detection, semiquantification, and clinical use of the Plasmodium falciparum histidine-rich protein-2 (PfHRP-2) as a parasite antigen biomarker for CM. The PfHRP-2 was detected in archival CSF samples from CM patients from Tanzania both by a newly developed sensitive and specific immuno-polymerase chain reaction (72 of 73) and by rapid diagnostic tests (62 of 73). The geometric mean PfHRP-2 CSF concentration was 8.76 ng/mL with no differences in those who survived (9.2 ng/mL), those who died (11.1 ng/mL), and those with neurologic sequelae (10.8 ng/mL). All aparasitemic endemic and nonendemic control samples had undetectable CSF PfHRP-2. In a separate group of 11 matched plasma and CSF cerebral malaria patient samples, the ratio of plasma to CSF PfHRP-2 was 175. The CSF PfHRP-2 reflects elevated plasma PfHRP-2 rather than elevated CM-specific CSF ratios, falling short of a validated biomarker.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Am J Trop Med Hyg

DOI

EISSN

1476-1645

Publication Date

September 2014

Volume

91

Issue

3

Start / End Page

486 / 492

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Tanzania
  • Sensitivity and Specificity
  • Reagent Kits, Diagnostic
  • Protozoan Proteins
  • Polymerase Chain Reaction
  • Plasmodium falciparum
  • Male
  • Malaria, Cerebral
  • Infant
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mikita, K., Thakur, K., Anstey, N. M., Piera, K. A., Pardo, C. A., Weinberg, J. B., … Sullivan, D. J. (2014). Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients. Am J Trop Med Hyg, 91(3), 486–492. https://doi.org/10.4269/ajtmh.14-0210
Mikita, Kei, Kiran Thakur, Nicholas M. Anstey, Kim A. Piera, Carlos A. Pardo, J Brice Weinberg, Jackson Mukemba, et al. “Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients.Am J Trop Med Hyg 91, no. 3 (September 2014): 486–92. https://doi.org/10.4269/ajtmh.14-0210.
Mikita K, Thakur K, Anstey NM, Piera KA, Pardo CA, Weinberg JB, et al. Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients. Am J Trop Med Hyg. 2014 Sep;91(3):486–92.
Mikita, Kei, et al. “Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients.Am J Trop Med Hyg, vol. 91, no. 3, Sept. 2014, pp. 486–92. Pubmed, doi:10.4269/ajtmh.14-0210.
Mikita K, Thakur K, Anstey NM, Piera KA, Pardo CA, Weinberg JB, Mukemba J, Florence S, Mwaikambo ED, Granger DL, Sullivan DJ. Quantification of Plasmodium falciparum histidine-rich protein-2 in cerebrospinal spinal fluid from cerebral malaria patients. Am J Trop Med Hyg. 2014 Sep;91(3):486–492.

Published In

Am J Trop Med Hyg

DOI

EISSN

1476-1645

Publication Date

September 2014

Volume

91

Issue

3

Start / End Page

486 / 492

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Tanzania
  • Sensitivity and Specificity
  • Reagent Kits, Diagnostic
  • Protozoan Proteins
  • Polymerase Chain Reaction
  • Plasmodium falciparum
  • Male
  • Malaria, Cerebral
  • Infant