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Vibrio metoecus sp. nov., a close relative of Vibrio cholerae isolated from coastal brackish ponds and clinical specimens.

Publication ,  Journal Article
Kirchberger, PC; Turnsek, M; Hunt, DE; Haley, BJ; Colwell, RR; Polz, MF; Tarr, CL; Boucher, Y
Published in: International journal of systematic and evolutionary microbiology
September 2014

A Gram-staining-negative, curved-rod-shaped bacterium with close resemblance to Vibrio cholerae, the aetiological agent of cholera, was isolated over the course of several years from coastal brackish water (17 strains) and from clinical cases (two strains) in the United States. 16S rRNA gene identity with V. cholerae exceeded 98 % yet an average nucleotide identity based on genome data of around 86 % and multi locus sequence analysis of six housekeeping genes (mdh, adk, gyrB, recA, pgi and rpoB) clearly delineated these isolates as a distinct genotypic cluster within the V. cholerae-V. mimicus clade. Most standard identification techniques do not differentiate this cluster of isolates from V. cholerae. Only amplification of the ompW gene using V. cholerae-specific primers and a negative Voges-Proskauer test showed a difference between the two clusters. Additionally, all isolated strains differed phenotypically from V. cholerae in their ability to utilize N-acetyl-d-galactosamine and d-glucuronic acid as sole carbon sources. Furthermore, they were generally unable to infect the slime mould Dictyostelium discoideum, a widespread ability in V. cholerae. Based on these clear phenotypic differences that are not necessarily apparent in standard tests as well as average nucleotide identity and phylogeny of protein-coding genes, we propose the existence of a novel species, Vibrio metoecus sp. nov. with the type strain OP3H(T) ( = LMG 27764(T) = CIP 110643(T)). Due to its close resemblance to V. cholerae and the increasing number of strains isolated over the past several years, we suggest that V. metoecus sp. nov. is a relatively common species of the genus Vibrio, isolates of which have been identified as atypical isolates of V. cholerae in the past. Its isolation from clinical samples also indicates that strains of this species, like V. cholerae, are opportunistic pathogens.

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Published In

International journal of systematic and evolutionary microbiology

DOI

EISSN

1466-5034

ISSN

1466-5026

Publication Date

September 2014

Volume

64

Issue

Pt 9

Start / End Page

3208 / 3214

Related Subject Headings

  • Vibrio
  • United States
  • Sequence Analysis, DNA
  • RNA, Ribosomal, 16S
  • Ponds
  • Phylogeny
  • Multilocus Sequence Typing
  • Molecular Sequence Data
  • Microbiology
  • Humans
 

Citation

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ICMJE
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Kirchberger, P. C., Turnsek, M., Hunt, D. E., Haley, B. J., Colwell, R. R., Polz, M. F., … Boucher, Y. (2014). Vibrio metoecus sp. nov., a close relative of Vibrio cholerae isolated from coastal brackish ponds and clinical specimens. International Journal of Systematic and Evolutionary Microbiology, 64(Pt 9), 3208–3214. https://doi.org/10.1099/ijs.0.060145-0
Kirchberger, Paul C., Maryann Turnsek, Dana E. Hunt, Bradd J. Haley, Rita R. Colwell, Martin F. Polz, Cheryl L. Tarr, and Yan Boucher. “Vibrio metoecus sp. nov., a close relative of Vibrio cholerae isolated from coastal brackish ponds and clinical specimens.International Journal of Systematic and Evolutionary Microbiology 64, no. Pt 9 (September 2014): 3208–14. https://doi.org/10.1099/ijs.0.060145-0.
Kirchberger PC, Turnsek M, Hunt DE, Haley BJ, Colwell RR, Polz MF, et al. Vibrio metoecus sp. nov., a close relative of Vibrio cholerae isolated from coastal brackish ponds and clinical specimens. International journal of systematic and evolutionary microbiology. 2014 Sep;64(Pt 9):3208–14.
Kirchberger, Paul C., et al. “Vibrio metoecus sp. nov., a close relative of Vibrio cholerae isolated from coastal brackish ponds and clinical specimens.International Journal of Systematic and Evolutionary Microbiology, vol. 64, no. Pt 9, Sept. 2014, pp. 3208–14. Epmc, doi:10.1099/ijs.0.060145-0.
Kirchberger PC, Turnsek M, Hunt DE, Haley BJ, Colwell RR, Polz MF, Tarr CL, Boucher Y. Vibrio metoecus sp. nov., a close relative of Vibrio cholerae isolated from coastal brackish ponds and clinical specimens. International journal of systematic and evolutionary microbiology. 2014 Sep;64(Pt 9):3208–3214.

Published In

International journal of systematic and evolutionary microbiology

DOI

EISSN

1466-5034

ISSN

1466-5026

Publication Date

September 2014

Volume

64

Issue

Pt 9

Start / End Page

3208 / 3214

Related Subject Headings

  • Vibrio
  • United States
  • Sequence Analysis, DNA
  • RNA, Ribosomal, 16S
  • Ponds
  • Phylogeny
  • Multilocus Sequence Typing
  • Molecular Sequence Data
  • Microbiology
  • Humans