The statistical conformation of a highly flexible protein: small-angle X-ray scattering of S. aureus protein A.
Staphylococcal protein A (SpA) is a multidomain protein consisting of five globular IgG binding domains separated by a conserved six- to nine-residue flexible linker. We collected SAXS data on the N-terminal protein-binding half of SpA (SpA-N) and constructs consisting of one to five domain modules in order to determine statistical conformation of this important S. aureus virulence factor. We fit the SAXS data to a scattering function based on a new polymer physics model, which provides an analytical description of the SpA-N statistical conformation. We describe a protocol for systematically determining the appropriate level of modeling to fit a SAXS data set based on goodness of fit and whether the addition of parameters improves it. In the case of SpA-N, the analytical polymer physics description provides a depiction of the statistical conformation of a flexible protein that, while lacking atomistic detail, properly reflects the information content of the data.
Duke Scholars
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- Staphylococcus aureus
- Staphylococcal Protein A
- Scattering, Small Angle
- Protein Conformation
- Molecular Sequence Data
- Molecular Biology
- Models, Molecular
- Biophysics
- Amino Acid Sequence
- 34 Chemical sciences
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Staphylococcus aureus
- Staphylococcal Protein A
- Scattering, Small Angle
- Protein Conformation
- Molecular Sequence Data
- Molecular Biology
- Models, Molecular
- Biophysics
- Amino Acid Sequence
- 34 Chemical sciences