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The statistical conformation of a highly flexible protein: small-angle X-ray scattering of S. aureus protein A.

Publication ,  Journal Article
Capp, JA; Hagarman, A; Richardson, DC; Oas, TG
Published in: Structure
August 5, 2014

Staphylococcal protein A (SpA) is a multidomain protein consisting of five globular IgG binding domains separated by a conserved six- to nine-residue flexible linker. We collected SAXS data on the N-terminal protein-binding half of SpA (SpA-N) and constructs consisting of one to five domain modules in order to determine statistical conformation of this important S. aureus virulence factor. We fit the SAXS data to a scattering function based on a new polymer physics model, which provides an analytical description of the SpA-N statistical conformation. We describe a protocol for systematically determining the appropriate level of modeling to fit a SAXS data set based on goodness of fit and whether the addition of parameters improves it. In the case of SpA-N, the analytical polymer physics description provides a depiction of the statistical conformation of a flexible protein that, while lacking atomistic detail, properly reflects the information content of the data.

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Published In

Structure

DOI

EISSN

1878-4186

Publication Date

August 5, 2014

Volume

22

Issue

8

Start / End Page

1184 / 1195

Location

United States

Related Subject Headings

  • Staphylococcus aureus
  • Staphylococcal Protein A
  • Scattering, Small Angle
  • Protein Conformation
  • Molecular Sequence Data
  • Molecular Biology
  • Models, Molecular
  • Biophysics
  • Amino Acid Sequence
  • 34 Chemical sciences
 

Citation

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Capp, J. A., Hagarman, A., Richardson, D. C., & Oas, T. G. (2014). The statistical conformation of a highly flexible protein: small-angle X-ray scattering of S. aureus protein A. Structure, 22(8), 1184–1195. https://doi.org/10.1016/j.str.2014.06.011
Capp, Jo A., Andrew Hagarman, David C. Richardson, and Terrence G. Oas. “The statistical conformation of a highly flexible protein: small-angle X-ray scattering of S. aureus protein A.Structure 22, no. 8 (August 5, 2014): 1184–95. https://doi.org/10.1016/j.str.2014.06.011.
Capp JA, Hagarman A, Richardson DC, Oas TG. The statistical conformation of a highly flexible protein: small-angle X-ray scattering of S. aureus protein A. Structure. 2014 Aug 5;22(8):1184–95.
Capp, Jo A., et al. “The statistical conformation of a highly flexible protein: small-angle X-ray scattering of S. aureus protein A.Structure, vol. 22, no. 8, Aug. 2014, pp. 1184–95. Pubmed, doi:10.1016/j.str.2014.06.011.
Capp JA, Hagarman A, Richardson DC, Oas TG. The statistical conformation of a highly flexible protein: small-angle X-ray scattering of S. aureus protein A. Structure. 2014 Aug 5;22(8):1184–1195.
Journal cover image

Published In

Structure

DOI

EISSN

1878-4186

Publication Date

August 5, 2014

Volume

22

Issue

8

Start / End Page

1184 / 1195

Location

United States

Related Subject Headings

  • Staphylococcus aureus
  • Staphylococcal Protein A
  • Scattering, Small Angle
  • Protein Conformation
  • Molecular Sequence Data
  • Molecular Biology
  • Models, Molecular
  • Biophysics
  • Amino Acid Sequence
  • 34 Chemical sciences