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Sequence analysis for full length genomes of human enterovirus 71 strains isolated in Linyi, Shandong Province

Publication ,  Journal Article
Wen, HL; Hao, SB; Gao, F; Zhao, L; Si, LY; Yuan, XJ; Wang, DX; Wang, ZY
Published in: Chinese Journal of Microbiology and Immunology (China)
July 1, 2011

Objective: To isolate enterovirus 71 from a death children, and analyze whether the neurovirulence was related to the variation of nucleotide and amino acid. Methods: Enterovirus 71 was isolated from throat swabs which were colleted from Shandong Linyi People's Hospital. The full length genome was sequenced by amplification with RT-PCR and sequencing of 9 overlapped gene fragments covering full length of the genomes. The nucleotide and amino acid sequenced was aligned by BLAST, Bioedit and MEGA 4. Results: A strain of enterovirus 71 was isolated and named as SDLY107. The full length was 7405 bp. The results of homology analysis of overall nucleotide sequence showed that strain Fuyang. Anhui. P. R. C/17.08/2 had highest homology (98.6%) with strain SDLY107, and the homology was 80.0% between strain SDLY107 with prototype strain BrCr/70, and 86.5% between strain SDLY107 with nerve strain MS/87. Phylogenetic analysis showed that the phylogeny was close between SDLY107 with some isolated strains from Chinese Mainland, such as Beijing, Henan, Guangxi, Shenzhen, Lanzhou, Fuyang, Chongqing and Zhejiang strains, which was clustered for C4 subtype. The results of amino acid sequence analysis showed that there were 2 mutations, E947D and K1873R, for strain SDLY107. Conclusion: SDLY107 belonged to C4 subtype, amino acid mutations E947D and K1873R of which may be relevant to the pathogenicity of EV71. Copyright © 2011 by the Chinese Medical Association.

Duke Scholars

Published In

Chinese Journal of Microbiology and Immunology (China)

DOI

ISSN

0254-5101

Publication Date

July 1, 2011

Volume

31

Issue

7

Start / End Page

603 / 608

Related Subject Headings

  • Virology
 

Citation

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Wen, H. L., Hao, S. B., Gao, F., Zhao, L., Si, L. Y., Yuan, X. J., … Wang, Z. Y. (2011). Sequence analysis for full length genomes of human enterovirus 71 strains isolated in Linyi, Shandong Province. Chinese Journal of Microbiology and Immunology (China), 31(7), 603–608. https://doi.org/10.3760/cma.j.issn.0254-5101.2011.07.007
Wen, H. L., S. B. Hao, F. Gao, L. Zhao, L. Y. Si, X. J. Yuan, D. X. Wang, and Z. Y. Wang. “Sequence analysis for full length genomes of human enterovirus 71 strains isolated in Linyi, Shandong Province.” Chinese Journal of Microbiology and Immunology (China) 31, no. 7 (July 1, 2011): 603–8. https://doi.org/10.3760/cma.j.issn.0254-5101.2011.07.007.
Wen HL, Hao SB, Gao F, Zhao L, Si LY, Yuan XJ, et al. Sequence analysis for full length genomes of human enterovirus 71 strains isolated in Linyi, Shandong Province. Chinese Journal of Microbiology and Immunology (China). 2011 Jul 1;31(7):603–8.
Wen, H. L., et al. “Sequence analysis for full length genomes of human enterovirus 71 strains isolated in Linyi, Shandong Province.” Chinese Journal of Microbiology and Immunology (China), vol. 31, no. 7, July 2011, pp. 603–08. Scopus, doi:10.3760/cma.j.issn.0254-5101.2011.07.007.
Wen HL, Hao SB, Gao F, Zhao L, Si LY, Yuan XJ, Wang DX, Wang ZY. Sequence analysis for full length genomes of human enterovirus 71 strains isolated in Linyi, Shandong Province. Chinese Journal of Microbiology and Immunology (China). 2011 Jul 1;31(7):603–608.

Published In

Chinese Journal of Microbiology and Immunology (China)

DOI

ISSN

0254-5101

Publication Date

July 1, 2011

Volume

31

Issue

7

Start / End Page

603 / 608

Related Subject Headings

  • Virology