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Inflammatory cytokines and cellular metabolites as synovial fluid biomarkers of posttraumatic ankle arthritis.

Publication ,  Journal Article
Adams, SB; Nettles, DL; Jones, LC; Miller, SD; Guyton, GP; Schon, LC
Published in: Foot Ankle Int
December 2014

BACKGROUND: There is a paucity of research on posttraumatic ankle arthritis (PTAA). We aimed to identify synovial fluid PTAA biomarkers using cytokine analysis and metabolic profiling. METHODS: Ankle joint synovial fluid was obtained from 20 patients with PTAA and 20 patients with no ankle pain and no radiographic evidence of ankle arthritis (control group). Synovial fluid samples were analyzed for IFN-γ, TNF-α, MIP-1β, MCP-1, IL-1β, IL-1Ra, IL-4, IL-6, IL-8, IL-10, IL-13, and IL-15 using ELISA and for more than 3000 metabolites using liquid and gas chromatography with mass spectroscopy. To compare presence of cytokines and metabolites between groups, t tests were used. Random forest analysis was performed on metabolites to determine whether control and PTAA samples could be differentiated based on metabolic profile. RESULTS: IL-1Ra, IL-6, IL-8, IL-10, IL-15, and MCP-1 were significantly elevated in the PTAA group. In addition, 107 metabolites in the PTAA group were significantly altered, including derangement in amino acid, carbohydrate, lipid, and energy metabolism, extracellular matrix turnover, and collagen degradation. Random forest analysis yielded a predictive accuracy of 90% when using the metabolic profiles to distinguish between control and PTAA samples. CONCLUSION: This study identified inflammatory cytokines and metabolites present in the synovial fluid of PTAA. CLINICAL RELEVANCE: Several of these entities have previously been implicated in rheumatoid arthritis and osteoarthritis of the knee, but many could potentially be used as novel biomarkers of PTAA. Most importantly, the findings suggest that metabolites could be used to distinguish synovial fluid from patients with PTAA.

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Published In

Foot Ankle Int

DOI

EISSN

1944-7876

Publication Date

December 2014

Volume

35

Issue

12

Start / End Page

1241 / 1249

Location

United States

Related Subject Headings

  • Wounds and Injuries
  • Synovial Fluid
  • Sensitivity and Specificity
  • Sampling Studies
  • Reference Values
  • Radiography
  • Osteoarthritis
  • Orthopedics
  • Middle Aged
  • Metabolome
 

Citation

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Adams, S. B., Nettles, D. L., Jones, L. C., Miller, S. D., Guyton, G. P., & Schon, L. C. (2014). Inflammatory cytokines and cellular metabolites as synovial fluid biomarkers of posttraumatic ankle arthritis. Foot Ankle Int, 35(12), 1241–1249. https://doi.org/10.1177/1071100714550652
Adams, Samuel B., Dana L. Nettles, Lynne C. Jones, Stuart D. Miller, Gregory P. Guyton, and Lew C. Schon. “Inflammatory cytokines and cellular metabolites as synovial fluid biomarkers of posttraumatic ankle arthritis.Foot Ankle Int 35, no. 12 (December 2014): 1241–49. https://doi.org/10.1177/1071100714550652.
Adams SB, Nettles DL, Jones LC, Miller SD, Guyton GP, Schon LC. Inflammatory cytokines and cellular metabolites as synovial fluid biomarkers of posttraumatic ankle arthritis. Foot Ankle Int. 2014 Dec;35(12):1241–9.
Adams, Samuel B., et al. “Inflammatory cytokines and cellular metabolites as synovial fluid biomarkers of posttraumatic ankle arthritis.Foot Ankle Int, vol. 35, no. 12, Dec. 2014, pp. 1241–49. Pubmed, doi:10.1177/1071100714550652.
Adams SB, Nettles DL, Jones LC, Miller SD, Guyton GP, Schon LC. Inflammatory cytokines and cellular metabolites as synovial fluid biomarkers of posttraumatic ankle arthritis. Foot Ankle Int. 2014 Dec;35(12):1241–1249.
Journal cover image

Published In

Foot Ankle Int

DOI

EISSN

1944-7876

Publication Date

December 2014

Volume

35

Issue

12

Start / End Page

1241 / 1249

Location

United States

Related Subject Headings

  • Wounds and Injuries
  • Synovial Fluid
  • Sensitivity and Specificity
  • Sampling Studies
  • Reference Values
  • Radiography
  • Osteoarthritis
  • Orthopedics
  • Middle Aged
  • Metabolome