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Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila.

Publication ,  Journal Article
Wells, AR; Zou, RS; Tulu, US; Sokolow, AC; Crawford, JM; Edwards, GS; Kiehart, DP
Published in: Molecular biology of the cell
November 2014

Drosophila's dorsal closure provides an excellent model system with which to analyze biomechanical processes during morphogenesis. During native closure, the amnioserosa, flanked by two lateral epidermal sheets, forms an eye-shaped opening with canthi at each corner. The dynamics of amnioserosa cells and actomyosin purse strings in the leading edges of epidermal cells promote closure, whereas the bulk of the lateral epidermis opposes closure. Canthi maintain purse string curvature (necessary for their dorsalward forces), and zipping at the canthi shortens leading edges, ensuring a continuous epithelium at closure completion. We investigated the requirement for intact canthi during closure with laser dissection approaches. Dissection of one or both canthi resulted in tissue recoil and flattening of each purse string. After recoil and a temporary pause, closure resumed at approximately native rates until slowing near the completion of closure. Thus the amnioserosa alone can drive closure after dissection of one or both canthi, requiring neither substantial purse string curvature nor zipping during the bulk of closure. How the embryo coordinates multiple, large forces (each of which is orders of magnitude greater than the net force) during native closure and is also resilient to multiple perturbations are key extant questions.

Duke Scholars

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

November 2014

Volume

25

Issue

22

Start / End Page

3552 / 3568

Related Subject Headings

  • Serous Membrane
  • Morphogenesis
  • Mechanotransduction, Cellular
  • Laser Capture Microdissection
  • Epithelial Cells
  • Epidermis
  • Embryonic Development
  • Embryo, Nonmammalian
  • Drosophila melanogaster
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wells, A. R., Zou, R. S., Tulu, U. S., Sokolow, A. C., Crawford, J. M., Edwards, G. S., & Kiehart, D. P. (2014). Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila. Molecular Biology of the Cell, 25(22), 3552–3568. https://doi.org/10.1091/mbc.e14-07-1190
Wells, Adrienne R., Roger S. Zou, U Serdar Tulu, Adam C. Sokolow, Janice M. Crawford, Glenn S. Edwards, and Daniel P. Kiehart. “Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila.Molecular Biology of the Cell 25, no. 22 (November 2014): 3552–68. https://doi.org/10.1091/mbc.e14-07-1190.
Wells AR, Zou RS, Tulu US, Sokolow AC, Crawford JM, Edwards GS, et al. Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila. Molecular biology of the cell. 2014 Nov;25(22):3552–68.
Wells, Adrienne R., et al. “Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila.Molecular Biology of the Cell, vol. 25, no. 22, Nov. 2014, pp. 3552–68. Epmc, doi:10.1091/mbc.e14-07-1190.
Wells AR, Zou RS, Tulu US, Sokolow AC, Crawford JM, Edwards GS, Kiehart DP. Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila. Molecular biology of the cell. 2014 Nov;25(22):3552–3568.

Published In

Molecular biology of the cell

DOI

EISSN

1939-4586

ISSN

1059-1524

Publication Date

November 2014

Volume

25

Issue

22

Start / End Page

3552 / 3568

Related Subject Headings

  • Serous Membrane
  • Morphogenesis
  • Mechanotransduction, Cellular
  • Laser Capture Microdissection
  • Epithelial Cells
  • Epidermis
  • Embryonic Development
  • Embryo, Nonmammalian
  • Drosophila melanogaster
  • Developmental Biology