Skip to main content
Journal cover image

Potent immune responses in rhesus macaques induced by nonviral delivery of a self-amplifying RNA vaccine expressing HIV type 1 envelope with a cationic nanoemulsion.

Publication ,  Journal Article
Bogers, WM; Oostermeijer, H; Mooij, P; Koopman, G; Verschoor, EJ; Davis, D; Ulmer, JB; Brito, LA; Cu, Y; Banerjee, K; Otten, GR; Burke, B ...
Published in: J Infect Dis
March 15, 2015

Self-amplifying messenger RNA (mRNA) of positive-strand RNA viruses are effective vectors for in situ expression of vaccine antigens and have potential as a new vaccine technology platform well suited for global health applications. The SAM vaccine platform is based on a synthetic, self-amplifying mRNA delivered by a nonviral delivery system. The safety and immunogenicity of an HIV SAM vaccine encoding a clade C envelope glycoprotein formulated with a cationic nanoemulsion (CNE) delivery system was evaluated in rhesus macaques. The HIV SAM vaccine induced potent cellular immune responses that were greater in magnitude than those induced by self-amplifying mRNA packaged in a viral replicon particle (VRP) or by a recombinant HIV envelope protein formulated with MF59 adjuvant, anti-envelope binding (including anti-V1V2), and neutralizing antibody responses that exceeded those induced by the VRP vaccine. These studies provide the first evidence in nonhuman primates that HIV vaccination with a relatively low dose (50 µg) of formulated self-amplifying mRNA is safe and immunogenic.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Infect Dis

DOI

EISSN

1537-6613

Publication Date

March 15, 2015

Volume

211

Issue

6

Start / End Page

947 / 955

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • RNA, Viral
  • Microbiology
  • Male
  • Macaca mulatta
  • Immunity, Cellular
  • HIV-1
  • HIV Infections
  • Emulsions
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bogers, W. M., Oostermeijer, H., Mooij, P., Koopman, G., Verschoor, E. J., Davis, D., … Barnett, S. W. (2015). Potent immune responses in rhesus macaques induced by nonviral delivery of a self-amplifying RNA vaccine expressing HIV type 1 envelope with a cationic nanoemulsion. J Infect Dis, 211(6), 947–955. https://doi.org/10.1093/infdis/jiu522
Bogers, Willy M., Herman Oostermeijer, Petra Mooij, Gerrit Koopman, Ernst J. Verschoor, David Davis, Jeffrey B. Ulmer, et al. “Potent immune responses in rhesus macaques induced by nonviral delivery of a self-amplifying RNA vaccine expressing HIV type 1 envelope with a cationic nanoemulsion.J Infect Dis 211, no. 6 (March 15, 2015): 947–55. https://doi.org/10.1093/infdis/jiu522.
Bogers WM, Oostermeijer H, Mooij P, Koopman G, Verschoor EJ, Davis D, et al. Potent immune responses in rhesus macaques induced by nonviral delivery of a self-amplifying RNA vaccine expressing HIV type 1 envelope with a cationic nanoemulsion. J Infect Dis. 2015 Mar 15;211(6):947–55.
Bogers, Willy M., et al. “Potent immune responses in rhesus macaques induced by nonviral delivery of a self-amplifying RNA vaccine expressing HIV type 1 envelope with a cationic nanoemulsion.J Infect Dis, vol. 211, no. 6, Mar. 2015, pp. 947–55. Pubmed, doi:10.1093/infdis/jiu522.
Bogers WM, Oostermeijer H, Mooij P, Koopman G, Verschoor EJ, Davis D, Ulmer JB, Brito LA, Cu Y, Banerjee K, Otten GR, Burke B, Dey A, Heeney JL, Shen X, Tomaras GD, Labranche C, Montefiori DC, Liao H-X, Haynes B, Geall AJ, Barnett SW. Potent immune responses in rhesus macaques induced by nonviral delivery of a self-amplifying RNA vaccine expressing HIV type 1 envelope with a cationic nanoemulsion. J Infect Dis. 2015 Mar 15;211(6):947–955.
Journal cover image

Published In

J Infect Dis

DOI

EISSN

1537-6613

Publication Date

March 15, 2015

Volume

211

Issue

6

Start / End Page

947 / 955

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • RNA, Viral
  • Microbiology
  • Male
  • Macaca mulatta
  • Immunity, Cellular
  • HIV-1
  • HIV Infections
  • Emulsions
  • Cells, Cultured