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SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases.

Publication ,  Journal Article
Yang, W; Paschen, W
Published in: Proteomics
March 2015

Small ubiquitin-like modifier (SUMO1-3) conjugation is a posttranslational protein modification whereby SUMOs are conjugated to lysine residues of target proteins. SUMO conjugation can alter the activity, stability, and function of target proteins, and thereby modulate almost all major cellular pathways. Many diseases are associated with SUMO conjugation, including heart failure, arthritis, cancer, degenerative diseases, and brain ischemia/stroke. It is, therefore, of major interest to characterize the SUMO-modified proteome regulated by these disorders. SUMO proteomics analysis is hampered by low levels of SUMOylated proteins. Several strategies have, therefore, been developed to enrich SUMOylated proteins from cell/tissue extracts. These include proteomics analysis on cells expressing epitope-tagged SUMO isoforms, use of monoclonal SUMO antibodies for immunoprecipitation and epitope-specific peptides for elution, and affinity purification with peptides containing SUMO interaction motifs to specifically enrich polySUMOylated proteins. Recently, two mouse models were generated and characterized that express tagged SUMO isoforms, and allow purification of SUMOylated proteins from complex organ extracts. Ultimately, these new analytical tools will help to decipher the SUMO-modified proteome regulated by various human diseases, and thereby, identify new targets for preventive and therapeutic purposes.

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Published In

Proteomics

DOI

EISSN

1615-9861

Publication Date

March 2015

Volume

15

Issue

5-6

Start / End Page

1181 / 1191

Location

Germany

Related Subject Headings

  • Sumoylation
  • Stroke
  • SUMO-1 Protein
  • Proteomics
  • Proteome
  • Neurodegenerative Diseases
  • Neoplasms
  • Mice
  • Humans
  • Heart Diseases
 

Citation

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MLA
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Yang, W., & Paschen, W. (2015). SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases. Proteomics, 15(5–6), 1181–1191. https://doi.org/10.1002/pmic.201400298
Yang, Wei, and Wulf Paschen. “SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases.Proteomics 15, no. 5–6 (March 2015): 1181–91. https://doi.org/10.1002/pmic.201400298.
Yang W, Paschen W. SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases. Proteomics. 2015 Mar;15(5–6):1181–91.
Yang, Wei, and Wulf Paschen. “SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases.Proteomics, vol. 15, no. 5–6, Mar. 2015, pp. 1181–91. Pubmed, doi:10.1002/pmic.201400298.
Yang W, Paschen W. SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases. Proteomics. 2015 Mar;15(5–6):1181–1191.
Journal cover image

Published In

Proteomics

DOI

EISSN

1615-9861

Publication Date

March 2015

Volume

15

Issue

5-6

Start / End Page

1181 / 1191

Location

Germany

Related Subject Headings

  • Sumoylation
  • Stroke
  • SUMO-1 Protein
  • Proteomics
  • Proteome
  • Neurodegenerative Diseases
  • Neoplasms
  • Mice
  • Humans
  • Heart Diseases