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Removable cranial windows for long-term imaging in awake mice.

Publication ,  Journal Article
Goldey, GJ; Roumis, DK; Glickfeld, LL; Kerlin, AM; Reid, RC; Bonin, V; Schafer, DP; Andermann, ML
Published in: Nat Protoc
November 2014

Cranial window implants in head-fixed rodents are becoming a preparation of choice for stable optical access to large areas of the cortex over extended periods of time. Here we provide a highly detailed and reliable surgical protocol for a cranial window implantation procedure for chronic wide-field and cellular imaging in awake, head-fixed mice, which enables subsequent window removal and replacement in the weeks and months after the initial craniotomy. This protocol has facilitated awake, chronic imaging in adolescent and adult mice over several months from a large number of cortical brain regions; targeted virus and tracer injections from data obtained using prior awake functional mapping; and functionally targeted two-photon imaging across all cortical layers in awake mice using a microprism attachment to the cranial window. Collectively, these procedures extend the reach of chronic imaging of cortical function and dysfunction in behaving animals.

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Published In

Nat Protoc

DOI

EISSN

1750-2799

Publication Date

November 2014

Volume

9

Issue

11

Start / End Page

2515 / 2538

Location

England

Related Subject Headings

  • Wakefulness
  • Skull
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Implants, Experimental
  • Equipment Design
  • Electroencephalography
  • Diagnostic Imaging
  • Craniotomy
  • Cerebral Cortex
 

Citation

APA
Chicago
ICMJE
MLA
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Goldey, G. J., Roumis, D. K., Glickfeld, L. L., Kerlin, A. M., Reid, R. C., Bonin, V., … Andermann, M. L. (2014). Removable cranial windows for long-term imaging in awake mice. Nat Protoc, 9(11), 2515–2538. https://doi.org/10.1038/nprot.2014.165
Goldey, Glenn J., Demetris K. Roumis, Lindsey L. Glickfeld, Aaron M. Kerlin, R Clay Reid, Vincent Bonin, Dorothy P. Schafer, and Mark L. Andermann. “Removable cranial windows for long-term imaging in awake mice.Nat Protoc 9, no. 11 (November 2014): 2515–38. https://doi.org/10.1038/nprot.2014.165.
Goldey GJ, Roumis DK, Glickfeld LL, Kerlin AM, Reid RC, Bonin V, et al. Removable cranial windows for long-term imaging in awake mice. Nat Protoc. 2014 Nov;9(11):2515–38.
Goldey, Glenn J., et al. “Removable cranial windows for long-term imaging in awake mice.Nat Protoc, vol. 9, no. 11, Nov. 2014, pp. 2515–38. Pubmed, doi:10.1038/nprot.2014.165.
Goldey GJ, Roumis DK, Glickfeld LL, Kerlin AM, Reid RC, Bonin V, Schafer DP, Andermann ML. Removable cranial windows for long-term imaging in awake mice. Nat Protoc. 2014 Nov;9(11):2515–2538.

Published In

Nat Protoc

DOI

EISSN

1750-2799

Publication Date

November 2014

Volume

9

Issue

11

Start / End Page

2515 / 2538

Location

England

Related Subject Headings

  • Wakefulness
  • Skull
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Implants, Experimental
  • Equipment Design
  • Electroencephalography
  • Diagnostic Imaging
  • Craniotomy
  • Cerebral Cortex