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Differential localization and potency of manganese porphyrin superoxide dismutase-mimicking compounds in Saccharomyces cerevisiae.

Publication ,  Journal Article
Li, AM; Martins, J; Tovmasyan, A; Valentine, JS; Batinic-Haberle, I; Spasojevic, I; Gralla, EB
Published in: Redox Biol
2014

Cationic Mn(III) porphyrin complexes based on MnTM-2-PyP are among the most promising superoxide dismutase (SOD) mimicking compounds being considered as potential anti-inflammatory drugs. We studied four of these active compounds in the yeast Saccharomyces cerevisiae, MnTM-2-PyP, MnTE-2-PyP, MnTnHex-2-PyP, and MnTnBu-2-PyP, each of which differs only in the length of its alkyl substituents. Each was active in improving the aerobic growth of yeast lacking SOD (sod1∆) in complete medium, and the efficacy of each mimic was correlated with its characteristic catalytic activity. We also studied the partitioning of these compounds between mitochondria and cytosol and found that the more hydrophobic members of the series accumulated in the mitochondria. Moreover, the degree to which a mimic mitigated the sod1Δ auxotrophic phenotype for lysine relative to its auxotrophic phenotype for methionine depended upon its level of lipophilicity-dependent accumulation inside the mitochondria. We conclude that localization within the cell is an important factor in biological efficacy in addition to the degree of catalytic activity, and we discuss possible explanations for this effect.

Duke Scholars

Published In

Redox Biol

DOI

EISSN

2213-2317

Publication Date

2014

Volume

3

Start / End Page

1 / 6

Location

Netherlands

Related Subject Headings

  • Superoxide Dismutase
  • Saccharomyces cerevisiae
  • Oxidation-Reduction
  • Molecular Mimicry
  • Mitochondria
  • Metalloporphyrins
  • Cytosol
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 1115 Pharmacology and Pharmaceutical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, A. M., Martins, J., Tovmasyan, A., Valentine, J. S., Batinic-Haberle, I., Spasojevic, I., & Gralla, E. B. (2014). Differential localization and potency of manganese porphyrin superoxide dismutase-mimicking compounds in Saccharomyces cerevisiae. Redox Biol, 3, 1–6. https://doi.org/10.1016/j.redox.2014.09.003
Li, Alice Ma, Jake Martins, Artak Tovmasyan, Joan S. Valentine, Ines Batinic-Haberle, Ivan Spasojevic, and Edith B. Gralla. “Differential localization and potency of manganese porphyrin superoxide dismutase-mimicking compounds in Saccharomyces cerevisiae.Redox Biol 3 (2014): 1–6. https://doi.org/10.1016/j.redox.2014.09.003.
Li AM, Martins J, Tovmasyan A, Valentine JS, Batinic-Haberle I, Spasojevic I, et al. Differential localization and potency of manganese porphyrin superoxide dismutase-mimicking compounds in Saccharomyces cerevisiae. Redox Biol. 2014;3:1–6.
Li, Alice Ma, et al. “Differential localization and potency of manganese porphyrin superoxide dismutase-mimicking compounds in Saccharomyces cerevisiae.Redox Biol, vol. 3, 2014, pp. 1–6. Pubmed, doi:10.1016/j.redox.2014.09.003.
Li AM, Martins J, Tovmasyan A, Valentine JS, Batinic-Haberle I, Spasojevic I, Gralla EB. Differential localization and potency of manganese porphyrin superoxide dismutase-mimicking compounds in Saccharomyces cerevisiae. Redox Biol. 2014;3:1–6.
Journal cover image

Published In

Redox Biol

DOI

EISSN

2213-2317

Publication Date

2014

Volume

3

Start / End Page

1 / 6

Location

Netherlands

Related Subject Headings

  • Superoxide Dismutase
  • Saccharomyces cerevisiae
  • Oxidation-Reduction
  • Molecular Mimicry
  • Mitochondria
  • Metalloporphyrins
  • Cytosol
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 1115 Pharmacology and Pharmaceutical Sciences