Skip to main content

Differing mechanisms for glutamate receptor aggregation on dendritic spines and shafts in cultured hippocampal neurons.

Publication ,  Journal Article
Mi, R; Tang, X; Sutter, R; Xu, D; Worley, P; O'Brien, RJ
Published in: J Neurosci
September 1, 2002

We have explored the ability of axons from spinal and hippocampal neurons to aggregate NMDA- and AMPA-type glutamate receptors on each other as a way of exploring the molecular differences between their presynaptic elements. Spinal axons, which normally cluster only AMPA-type glutamate receptors on other spinal neurons, cluster both AMPA- and NMDA-type glutamate receptors on the dendritic shafts of hippocampal interneurons but are ineffective at clustering either subtype of glutamate receptor on the dendritic spines of hippocampal pyramidal neurons. Conversely, hippocampal axons appear to be multipotent, capable of clustering both AMPA- and NMDA-type glutamate receptors on hippocampal interneurons and pyramidal cells. The secretion of the neuronal activity-regulated pentraxin (Narp) by hippocampal axons is restricted to contacts with interneurons. Exogenous application of Narp to cultured hippocampal neurons results in clusters of both NMDA- and AMPA-type glutamate receptors on hippocampal interneurons but not hippocampal pyramidal neurons. Because Narp displays no ability to directly aggregate NMDA receptors, we propose that Narp aggregates NMDA receptors in hippocampal interneurons indirectly through cytoplasmic coupling to synaptic AMPA receptors. Furthermore, our data suggest the existence of a novel molecule(s), capable of forming excitatory synapses on dendritic spines.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

September 1, 2002

Volume

22

Issue

17

Start / End Page

7606 / 7616

Location

United States

Related Subject Headings

  • Transfection
  • Spinal Cord
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Glutamate
  • Receptors, AMPA
  • Receptor Aggregation
  • Rats, Sprague-Dawley
  • Rats
  • Pyramidal Cells
  • Neurons
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mi, R., Tang, X., Sutter, R., Xu, D., Worley, P., & O’Brien, R. J. (2002). Differing mechanisms for glutamate receptor aggregation on dendritic spines and shafts in cultured hippocampal neurons. J Neurosci, 22(17), 7606–7616. https://doi.org/10.1523/JNEUROSCI.22-17-07606.2002
Mi, Ruifa, Xiaopei Tang, Ralph Sutter, Desheng Xu, Paul Worley, and Richard J. O’Brien. “Differing mechanisms for glutamate receptor aggregation on dendritic spines and shafts in cultured hippocampal neurons.J Neurosci 22, no. 17 (September 1, 2002): 7606–16. https://doi.org/10.1523/JNEUROSCI.22-17-07606.2002.
Mi R, Tang X, Sutter R, Xu D, Worley P, O’Brien RJ. Differing mechanisms for glutamate receptor aggregation on dendritic spines and shafts in cultured hippocampal neurons. J Neurosci. 2002 Sep 1;22(17):7606–16.
Mi, Ruifa, et al. “Differing mechanisms for glutamate receptor aggregation on dendritic spines and shafts in cultured hippocampal neurons.J Neurosci, vol. 22, no. 17, Sept. 2002, pp. 7606–16. Pubmed, doi:10.1523/JNEUROSCI.22-17-07606.2002.
Mi R, Tang X, Sutter R, Xu D, Worley P, O’Brien RJ. Differing mechanisms for glutamate receptor aggregation on dendritic spines and shafts in cultured hippocampal neurons. J Neurosci. 2002 Sep 1;22(17):7606–7616.

Published In

J Neurosci

DOI

EISSN

1529-2401

Publication Date

September 1, 2002

Volume

22

Issue

17

Start / End Page

7606 / 7616

Location

United States

Related Subject Headings

  • Transfection
  • Spinal Cord
  • Receptors, N-Methyl-D-Aspartate
  • Receptors, Glutamate
  • Receptors, AMPA
  • Receptor Aggregation
  • Rats, Sprague-Dawley
  • Rats
  • Pyramidal Cells
  • Neurons