Skip to main content

Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol.

Publication ,  Journal Article
Ghosh, M; Roy, K; Das Mukherjee, D; Chakrabarti, G; Roy Choudhury, K; Roy, S
Published in: PLoS Negl Trop Dis
December 2014

BACKGROUND: The protozoan parasite Leishmania donovani (LD) reduces cellular cholesterol of the host possibly for its own benefit. Cholesterol is mostly present in the specialized compartment of the plasma membrane. The relation between mobility of membrane proteins and cholesterol depletion from membrane continues to be an important issue. The notion that leishmania infection alters the mobility of membrane proteins stems from our previous study where we showed that the distance between subunits of IFNγ receptor (R1 and R2) on the cell surface of LD infected cell is increased, but is restored to normal by liposomal cholesterol treatment. METHODOLOGY/PRINCIPAL FINDINGS: We determined the lateral mobility of a membrane protein in normal, LD infected and liposome treated LD infected cells using GFP-tagged PLCδ1 as a probe. The mobility of PLCδ1 was computationally analyzed from the time lapse experiment using boundary distance plot and radial profile movement. Our results showed that the lateral mobility of the membrane protein, which is increased in infection, is restored to normal upon liposomal cholesterol treatment. The results of FRAP experiment lent further credence to the above notion. The membrane proteins are intimately linked with cellular actin and alteration of cellular actin may influence lateral mobility. We found that F-actin is decreased in infection but is restored to normal upon liposomal cholesterol treatment as evident from phalloidin staining and also from biochemical analysis by immunoblotting. CONCLUSIONS/SIGNIFICANCES: To our knowledge this is the first direct demonstration that LD parasites during their intracellular life cycle increases lateral mobility of membrane proteins and decreases F-actin level in infected macrophages. Such defects may contribute to ineffective intracellular signaling and other cellular functions.

Duke Scholars

Published In

PLoS Negl Trop Dis

DOI

EISSN

1935-2735

Publication Date

December 2014

Volume

8

Issue

12

Start / End Page

e3367

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Mice
  • Membrane Proteins
  • Macrophages
  • Liposomes
  • Leishmaniasis, Visceral
  • Leishmania donovani
  • Cholesterol
  • Cell Line
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Ghosh, M., Roy, K., Das Mukherjee, D., Chakrabarti, G., Roy Choudhury, K., & Roy, S. (2014). Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. PLoS Negl Trop Dis, 8(12), e3367. https://doi.org/10.1371/journal.pntd.0003367
Ghosh, Moumita, Koushik Roy, Dipanwita Das Mukherjee, Gopal Chakrabarti, Kingshuk Roy Choudhury, and Syamal Roy. “Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol.PLoS Negl Trop Dis 8, no. 12 (December 2014): e3367. https://doi.org/10.1371/journal.pntd.0003367.
Ghosh M, Roy K, Das Mukherjee D, Chakrabarti G, Roy Choudhury K, Roy S. Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. PLoS Negl Trop Dis. 2014 Dec;8(12):e3367.
Ghosh, Moumita, et al. “Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol.PLoS Negl Trop Dis, vol. 8, no. 12, Dec. 2014, p. e3367. Pubmed, doi:10.1371/journal.pntd.0003367.
Ghosh M, Roy K, Das Mukherjee D, Chakrabarti G, Roy Choudhury K, Roy S. Leishmania donovani infection enhances lateral mobility of macrophage membrane protein which is reversed by liposomal cholesterol. PLoS Negl Trop Dis. 2014 Dec;8(12):e3367.

Published In

PLoS Negl Trop Dis

DOI

EISSN

1935-2735

Publication Date

December 2014

Volume

8

Issue

12

Start / End Page

e3367

Location

United States

Related Subject Headings

  • Tropical Medicine
  • Mice
  • Membrane Proteins
  • Macrophages
  • Liposomes
  • Leishmaniasis, Visceral
  • Leishmania donovani
  • Cholesterol
  • Cell Line
  • Animals