Development of a fluorometric microtiter plate-based enzyme assay for arylsulfatase B (MPS VI) using dried blood spots.
Publication
, Journal Article
Ullal, AJ; Millington, DS; Bali, DS
Published in: Mol Genet Metab Rep
2014
Mucopolysaccharidosis type VI or Maroteaux-Lamy syndrome is an autosomal recessive lysosomal storage disorder caused by deficiency of arylsulfatase B (ARS-B) enzyme activity. It results in mild to severe multi-organ system failure from accumulation of undigested glycosaminoglycans (GAGs); dermatan sulfate and chondroitin-4-sulfate. We have developed a single-step enzyme assay using a fluorescent substrate and dried blood spots to measure ARS-B activity to identify disease patients. This assay is robust, reproducible, specific and convenient to perform.
Duke Scholars
Published In
Mol Genet Metab Rep
DOI
ISSN
2214-4269
Publication Date
2014
Volume
1
Start / End Page
465 / 467
Location
United States
Related Subject Headings
- 3202 Clinical sciences
- 3105 Genetics
- 0604 Genetics
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Ullal, A. J., Millington, D. S., & Bali, D. S. (2014). Development of a fluorometric microtiter plate-based enzyme assay for arylsulfatase B (MPS VI) using dried blood spots. Mol Genet Metab Rep, 1, 465–467. https://doi.org/10.1016/j.ymgmr.2014.10.005
Ullal, Anirudh J., David S. Millington, and Deeksha S. Bali. “Development of a fluorometric microtiter plate-based enzyme assay for arylsulfatase B (MPS VI) using dried blood spots.” Mol Genet Metab Rep 1 (2014): 465–67. https://doi.org/10.1016/j.ymgmr.2014.10.005.
Ullal AJ, Millington DS, Bali DS. Development of a fluorometric microtiter plate-based enzyme assay for arylsulfatase B (MPS VI) using dried blood spots. Mol Genet Metab Rep. 2014;1:465–7.
Ullal, Anirudh J., et al. “Development of a fluorometric microtiter plate-based enzyme assay for arylsulfatase B (MPS VI) using dried blood spots.” Mol Genet Metab Rep, vol. 1, 2014, pp. 465–67. Pubmed, doi:10.1016/j.ymgmr.2014.10.005.
Ullal AJ, Millington DS, Bali DS. Development of a fluorometric microtiter plate-based enzyme assay for arylsulfatase B (MPS VI) using dried blood spots. Mol Genet Metab Rep. 2014;1:465–467.
Published In
Mol Genet Metab Rep
DOI
ISSN
2214-4269
Publication Date
2014
Volume
1
Start / End Page
465 / 467
Location
United States
Related Subject Headings
- 3202 Clinical sciences
- 3105 Genetics
- 0604 Genetics
- 0601 Biochemistry and Cell Biology