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High-frequency alloreactive T cells augment effector function of low-frequency CD8+ T-cell responses under CD28/CD154 blockade.

Publication ,  Journal Article
Floyd, TL; Orr, SB; Coley, SM; Hanna, SS; Wagener, ME; Kirk, AD; Larsen, CP; Ford, ML
Published in: Transplantation
May 27, 2010

BACKGROUND: Blockade of costimulatory molecules is a potent method of inducing long-term graft survival. We have previously addressed the issue of donor-reactive T-cell precursor frequency on relative costimulation dependence and found that the presence of a high precursor frequency of donor-reactive CD8 T cells resulted in costimulation blockade-resistant graft rejection, whereas the presence of a low-frequency donor-reactive population did not. To address the mechanisms by which high-frequency T cells obviated the requirement for costimulation, we asked whether a low-frequency population responding concomitantly with a high-frequency response also demonstrated costimulation independence. METHODS: A model system was established in which B6 mice containing a low frequency of anti-membrane bound chicken ovalbumin (mOVA) responders and a high frequency of anti-BALB/c responders received a skin graft from B6.mOVAxBALB/c F1 donors in the presence or absence of cytotoxic T-lymphocyte antigen-4 Ig/anti-CD154 costimulatory blockade. RESULTS: The results revealed that in the presence of costimulation blockade, high-frequency anti-BALB/c T cells augmented the effector activity of low-frequency anti-mOVA T cells, but it did not enhance the accumulation of anti-mOVA T cells capable of mediating graft rejection. CONCLUSIONS: These results demonstrate that both antigen-specific and antigen-independent factors contribute to the relative costimulation independence of high-frequency T-cell responses.

Duke Scholars

Published In

Transplantation

DOI

EISSN

1534-6080

Publication Date

May 27, 2010

Volume

89

Issue

10

Start / End Page

1208 / 1217

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Transcriptional Activation
  • T-Lymphocytes
  • Surgery
  • Skin Transplantation
  • Ovalbumin
  • Mice, Inbred Strains
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
 

Citation

APA
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ICMJE
MLA
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Floyd, T. L., Orr, S. B., Coley, S. M., Hanna, S. S., Wagener, M. E., Kirk, A. D., … Ford, M. L. (2010). High-frequency alloreactive T cells augment effector function of low-frequency CD8+ T-cell responses under CD28/CD154 blockade. Transplantation, 89(10), 1208–1217. https://doi.org/10.1097/TP.0b013e3181df53dc
Floyd, Tamara L., Steven B. Orr, Shana M. Coley, Samantha S. Hanna, Maylene E. Wagener, Allan D. Kirk, Christian P. Larsen, and Mandy L. Ford. “High-frequency alloreactive T cells augment effector function of low-frequency CD8+ T-cell responses under CD28/CD154 blockade.Transplantation 89, no. 10 (May 27, 2010): 1208–17. https://doi.org/10.1097/TP.0b013e3181df53dc.
Floyd TL, Orr SB, Coley SM, Hanna SS, Wagener ME, Kirk AD, et al. High-frequency alloreactive T cells augment effector function of low-frequency CD8+ T-cell responses under CD28/CD154 blockade. Transplantation. 2010 May 27;89(10):1208–17.
Floyd, Tamara L., et al. “High-frequency alloreactive T cells augment effector function of low-frequency CD8+ T-cell responses under CD28/CD154 blockade.Transplantation, vol. 89, no. 10, May 2010, pp. 1208–17. Pubmed, doi:10.1097/TP.0b013e3181df53dc.
Floyd TL, Orr SB, Coley SM, Hanna SS, Wagener ME, Kirk AD, Larsen CP, Ford ML. High-frequency alloreactive T cells augment effector function of low-frequency CD8+ T-cell responses under CD28/CD154 blockade. Transplantation. 2010 May 27;89(10):1208–1217.

Published In

Transplantation

DOI

EISSN

1534-6080

Publication Date

May 27, 2010

Volume

89

Issue

10

Start / End Page

1208 / 1217

Location

United States

Related Subject Headings

  • Transplantation, Homologous
  • Transcriptional Activation
  • T-Lymphocytes
  • Surgery
  • Skin Transplantation
  • Ovalbumin
  • Mice, Inbred Strains
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice