Skip to main content

Tumor necrosis factor-alpha and tumor growth factor-beta1 genotype: partial association with intragraft gene expression in two cases of long-term peripheral tolerance to a kidney transplant.

Publication ,  Journal Article
Burlingham, WJ; O'Connell, PJ; Jacobson, LM; Becker, BN; Kirk, AD; Pravica, V; Hutchinson, IV
Published in: Transplantation
April 15, 2000

Genomic DNA was obtained from peripheral blood samples of patients JB and DS each of whom received a kidney transplant at 16 years of age from a serologically HLA-DR matched and HLA-class I -mismatched donor. Both patients discontinued immunosuppression after 1-2 years and retained good renal function for an additional 5 years or more. DNA was analyzed for genetic polymorphisms in the tumor necrosis factor-alpha (TNFalpha) and tumor growth factor-beta1 (TGFbeta1) loci. Biopsy samples obtained during stable function (DS, JB) and during rejection (JB) were analyzed by RT/PCR for cytokine gene expression. Both patients had a high responder genotype for TGFbeta1. DS had a low responder TNFalpha genotype, while JB and his donor were both genotypically TNFalpha intermediate responders. DS had a high TGFbeta1: TNFalpha mRNA ratio in two biopsies obtained during tolerance, while JB, who eventually lost his graft, had more TNFalpha than TGFbeta1 mRNA. The results suggest a possible role for cytokine immunogenetics in the stability of peripheral tolerance.

Duke Scholars

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

April 15, 2000

Volume

69

Issue

7

Start / End Page

1527 / 1530

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Transforming Growth Factor beta
  • Time Factors
  • Surgery
  • RNA, Messenger
  • Kidney Transplantation
  • Kidney
  • Immune Tolerance
  • Humans
  • Genotype
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Burlingham, W. J., O’Connell, P. J., Jacobson, L. M., Becker, B. N., Kirk, A. D., Pravica, V., & Hutchinson, I. V. (2000). Tumor necrosis factor-alpha and tumor growth factor-beta1 genotype: partial association with intragraft gene expression in two cases of long-term peripheral tolerance to a kidney transplant. Transplantation, 69(7), 1527–1530. https://doi.org/10.1097/00007890-200004150-00058
Burlingham, W. J., P. J. O’Connell, L. M. Jacobson, B. N. Becker, A. D. Kirk, V. Pravica, and I. V. Hutchinson. “Tumor necrosis factor-alpha and tumor growth factor-beta1 genotype: partial association with intragraft gene expression in two cases of long-term peripheral tolerance to a kidney transplant.Transplantation 69, no. 7 (April 15, 2000): 1527–30. https://doi.org/10.1097/00007890-200004150-00058.
Burlingham WJ, O’Connell PJ, Jacobson LM, Becker BN, Kirk AD, Pravica V, et al. Tumor necrosis factor-alpha and tumor growth factor-beta1 genotype: partial association with intragraft gene expression in two cases of long-term peripheral tolerance to a kidney transplant. Transplantation. 2000 Apr 15;69(7):1527–30.
Burlingham, W. J., et al. “Tumor necrosis factor-alpha and tumor growth factor-beta1 genotype: partial association with intragraft gene expression in two cases of long-term peripheral tolerance to a kidney transplant.Transplantation, vol. 69, no. 7, Apr. 2000, pp. 1527–30. Pubmed, doi:10.1097/00007890-200004150-00058.
Burlingham WJ, O’Connell PJ, Jacobson LM, Becker BN, Kirk AD, Pravica V, Hutchinson IV. Tumor necrosis factor-alpha and tumor growth factor-beta1 genotype: partial association with intragraft gene expression in two cases of long-term peripheral tolerance to a kidney transplant. Transplantation. 2000 Apr 15;69(7):1527–1530.

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

April 15, 2000

Volume

69

Issue

7

Start / End Page

1527 / 1530

Location

United States

Related Subject Headings

  • Tumor Necrosis Factor-alpha
  • Transforming Growth Factor beta
  • Time Factors
  • Surgery
  • RNA, Messenger
  • Kidney Transplantation
  • Kidney
  • Immune Tolerance
  • Humans
  • Genotype