Skip to main content
Journal cover image

Use of donor serum to prevent passive transfer of hyperacute rejection.

Publication ,  Journal Article
Wang, J; Geissler, EK; Fechner, JH; Burlingham, WJ; Knechtle, SJ
Published in: J Surg Res
July 1994

Organ transplantation in presensitized recipients continues to be contraindicated for heart and kidney recipients due to the risk of hyperacute rejection, which has no known treatment at this time. We tested whether donor serum, which contains soluble MHC class I antigen, is able to neutralize the effect of anti-donor antibody in the recipient and prevent hyperacute or accelerated rejection. A rat model of passive immunization was used to test the role of anti-donor antibody in hyperacute rejection. Seven of 10 recipients of hyperimmune serum (HyS), derived from Lewis rats (RT1l) following 3 ACI (RT1a) skin grafts, developed hyperacute or accelerated rejection. Intravenous injection of ACI serum prior to the HyS administration prevented hyperacute rejection in all recipients tested. When third-party (Wistar-Furth, RT1u) serum was given to Lewis rats injected with HyS, hyperacute rejection was not abrogated. When examining the mechanism of this effect, a simple antibody blocking phenomenon was found to be unlikely since flow cytometry analysis showed that ACI serum needed to be present at > or = 256-fold excess compared to HyS to block anti-ACI antibody binding to RT1.Aa+cells by 50%. We tested whether the RT1.Aa class I antigen in ACI serum had other biologic properties that resulted in the prolonged graft survival. However, removal of RT1.Aa antigen from ACI serum prior to use in the passive transfer model did not abrogate the graft prolongation observed previously. These data suggest that components of donor serum other than MHC class I antigen may be useful for preventing the antibody-mediated component of hyperacute rejection.

Duke Scholars

Published In

J Surg Res

DOI

ISSN

0022-4804

Publication Date

July 1994

Volume

57

Issue

1

Start / End Page

150 / 155

Location

United States

Related Subject Headings

  • Tissue Donors
  • Time Factors
  • Surgery
  • Rats, Inbred Lew
  • Rats, Inbred ACI
  • Rats
  • Myocardium
  • Male
  • Immunization, Passive
  • Histocompatibility Antigens Class I
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, J., Geissler, E. K., Fechner, J. H., Burlingham, W. J., & Knechtle, S. J. (1994). Use of donor serum to prevent passive transfer of hyperacute rejection. J Surg Res, 57(1), 150–155. https://doi.org/10.1006/jsre.1994.1123
Wang, J., E. K. Geissler, J. H. Fechner, W. J. Burlingham, and S. J. Knechtle. “Use of donor serum to prevent passive transfer of hyperacute rejection.J Surg Res 57, no. 1 (July 1994): 150–55. https://doi.org/10.1006/jsre.1994.1123.
Wang J, Geissler EK, Fechner JH, Burlingham WJ, Knechtle SJ. Use of donor serum to prevent passive transfer of hyperacute rejection. J Surg Res. 1994 Jul;57(1):150–5.
Wang, J., et al. “Use of donor serum to prevent passive transfer of hyperacute rejection.J Surg Res, vol. 57, no. 1, July 1994, pp. 150–55. Pubmed, doi:10.1006/jsre.1994.1123.
Wang J, Geissler EK, Fechner JH, Burlingham WJ, Knechtle SJ. Use of donor serum to prevent passive transfer of hyperacute rejection. J Surg Res. 1994 Jul;57(1):150–155.
Journal cover image

Published In

J Surg Res

DOI

ISSN

0022-4804

Publication Date

July 1994

Volume

57

Issue

1

Start / End Page

150 / 155

Location

United States

Related Subject Headings

  • Tissue Donors
  • Time Factors
  • Surgery
  • Rats, Inbred Lew
  • Rats, Inbred ACI
  • Rats
  • Myocardium
  • Male
  • Immunization, Passive
  • Histocompatibility Antigens Class I