Skip to main content
Journal cover image

Human aqueous humor exosomes.

Publication ,  Journal Article
Dismuke, WM; Challa, P; Navarro, I; Stamer, WD; Liu, Y
Published in: Exp Eye Res
March 2015

Aqueous humor (AH) is a dynamic intraocular fluid that supports the vitality of tissues that regulate intraocular pressure. We recently discovered that extracellular nanovesicles called exosomes are a major constituent of AH. Exosomes function in extracellular communication and contain proteins and small RNA. Our goal was to characterize the physical properties of AH exosomes and their exosomal RNA (esRNA) content. We isolated exosomes from human AH collected during cataract surgery from five patients using serial ultracentrifugation. We measured the size and concentration of AH exosomes in solution using nanoparticle tracking analysis. We found a single population of vesicles having a mean size of 121 ± 11 nm in the unprocessed AH. Data show that centrifugation does not significantly affect the mean particle size (121 ± 11 nm versus 124 ± 21 nm), but does impact the final number of exosomes in solution (87% loss from the unprocessed AH; n = 5). We extracted esRNA from the pooled human AH samples using miRCURY RNA isolation kit from Exiqon. The quality of extracted esRNA was evaluated using Agilent Bioanalyzer 2100 and was used to generate a sequencing library for small RNA sequencing with Illumina MiSeq sequencer. More than 10 different miRNAs were identified; abundant species included miR-486-5p, miR-204, and miR-184. We found that the majority of extracellular vesicles in the AH were in the exosome size range, suggesting that miRNAs housed within exosomes may function in communication between AH inflow and outflow tissues.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

March 2015

Volume

132

Start / End Page

73 / 77

Location

England

Related Subject Headings

  • Ultracentrifugation
  • Ophthalmology & Optometry
  • MicroRNAs
  • Male
  • Humans
  • Female
  • Exosomes
  • Aqueous Humor
  • 3212 Ophthalmology and optometry
  • 1113 Opthalmology and Optometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dismuke, W. M., Challa, P., Navarro, I., Stamer, W. D., & Liu, Y. (2015). Human aqueous humor exosomes. Exp Eye Res, 132, 73–77. https://doi.org/10.1016/j.exer.2015.01.019
Dismuke, W Michael, Pratap Challa, Iris Navarro, W Daniel Stamer, and Yutao Liu. “Human aqueous humor exosomes.Exp Eye Res 132 (March 2015): 73–77. https://doi.org/10.1016/j.exer.2015.01.019.
Dismuke WM, Challa P, Navarro I, Stamer WD, Liu Y. Human aqueous humor exosomes. Exp Eye Res. 2015 Mar;132:73–7.
Dismuke, W. Michael, et al. “Human aqueous humor exosomes.Exp Eye Res, vol. 132, Mar. 2015, pp. 73–77. Pubmed, doi:10.1016/j.exer.2015.01.019.
Dismuke WM, Challa P, Navarro I, Stamer WD, Liu Y. Human aqueous humor exosomes. Exp Eye Res. 2015 Mar;132:73–77.
Journal cover image

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

March 2015

Volume

132

Start / End Page

73 / 77

Location

England

Related Subject Headings

  • Ultracentrifugation
  • Ophthalmology & Optometry
  • MicroRNAs
  • Male
  • Humans
  • Female
  • Exosomes
  • Aqueous Humor
  • 3212 Ophthalmology and optometry
  • 1113 Opthalmology and Optometry