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Angiopoietin-1 enhances skeletal muscle regeneration in mice.

Publication ,  Journal Article
Mofarrahi, M; McClung, JM; Kontos, CD; Davis, EC; Tappuni, B; Moroz, N; Pickett, AE; Huck, L; Harel, S; Danialou, G; Hussain, SNA
Published in: Am J Physiol Regul Integr Comp Physiol
April 1, 2015

Activation of muscle progenitor cell myogenesis and endothelial cell angiogenesis is critical for the recovery of skeletal muscle from injury. Angiopoietin-1 (Ang-1), a ligand of Tie-2 receptors, enhances angiogenesis and skeletal muscle satellite cell survival; however, its role in skeletal muscle regeneration after injury is unknown. We assessed the effects of Ang-1 on fiber regeneration, myogenesis, and angiogenesis in injured skeletal muscle (tibialis anterior, TA) in mice. We also assessed endogenous Ang-1 levels and localization in intact and injured TA muscles. TA fiber injury was triggered by cardiotoxin injection. Endogenous Ang-1 mRNA levels immediately decreased in response to cardiotoxin then increased during the 2 wk. Ang-1 protein was expressed in satellite cells, both in noninjured and recovering TA muscles. Positive Ang-1 staining was present in blood vessels but not in nerve fibers. Four days after the initiation of injury, injection of adenoviral Ang-1 into injured muscles resulted in significant increases in in situ TA muscle contractility, muscle fiber regeneration, and capillary density. In cultured human skeletal myoblasts, recombinant Ang-1 protein increased survival, proliferation, migration, and differentiation into myotubes. The latter effect was associated with significant upregulation of the expression of the myogenic regulatory factors MyoD and Myogenin and certain genes involved in cell cycle regulation. We conclude that Ang-1 strongly enhances skeletal muscle regeneration in response to fiber injury and that this effect is mediated through induction of the myogenesis program in muscle progenitor cells and the angiogenesis program in endothelial cells.

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Published In

Am J Physiol Regul Integr Comp Physiol

DOI

EISSN

1522-1490

Publication Date

April 1, 2015

Volume

308

Issue

7

Start / End Page

R576 / R589

Location

United States

Related Subject Headings

  • Time Factors
  • Signal Transduction
  • Regeneration
  • RNA, Messenger
  • Physiology
  • Necrosis
  • Myoblasts
  • Muscular Diseases
  • Muscle, Skeletal
  • Muscle Development
 

Citation

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Mofarrahi, M., McClung, J. M., Kontos, C. D., Davis, E. C., Tappuni, B., Moroz, N., … Hussain, S. N. A. (2015). Angiopoietin-1 enhances skeletal muscle regeneration in mice. Am J Physiol Regul Integr Comp Physiol, 308(7), R576–R589. https://doi.org/10.1152/ajpregu.00267.2014
Mofarrahi, Mahroo, Joseph M. McClung, Christopher D. Kontos, Elaine C. Davis, Bassman Tappuni, Nicolay Moroz, Amy E. Pickett, et al. “Angiopoietin-1 enhances skeletal muscle regeneration in mice.Am J Physiol Regul Integr Comp Physiol 308, no. 7 (April 1, 2015): R576–89. https://doi.org/10.1152/ajpregu.00267.2014.
Mofarrahi M, McClung JM, Kontos CD, Davis EC, Tappuni B, Moroz N, et al. Angiopoietin-1 enhances skeletal muscle regeneration in mice. Am J Physiol Regul Integr Comp Physiol. 2015 Apr 1;308(7):R576–89.
Mofarrahi, Mahroo, et al. “Angiopoietin-1 enhances skeletal muscle regeneration in mice.Am J Physiol Regul Integr Comp Physiol, vol. 308, no. 7, Apr. 2015, pp. R576–89. Pubmed, doi:10.1152/ajpregu.00267.2014.
Mofarrahi M, McClung JM, Kontos CD, Davis EC, Tappuni B, Moroz N, Pickett AE, Huck L, Harel S, Danialou G, Hussain SNA. Angiopoietin-1 enhances skeletal muscle regeneration in mice. Am J Physiol Regul Integr Comp Physiol. 2015 Apr 1;308(7):R576–R589.

Published In

Am J Physiol Regul Integr Comp Physiol

DOI

EISSN

1522-1490

Publication Date

April 1, 2015

Volume

308

Issue

7

Start / End Page

R576 / R589

Location

United States

Related Subject Headings

  • Time Factors
  • Signal Transduction
  • Regeneration
  • RNA, Messenger
  • Physiology
  • Necrosis
  • Myoblasts
  • Muscular Diseases
  • Muscle, Skeletal
  • Muscle Development