Methods to generate genetically engineered mouse models of soft tissue sarcoma.
Publication
, Journal Article
Dodd, RD; Añó, L; Blum, JM; Li, Z; Van Mater, D; Kirsch, DG
Published in: Methods Mol Biol
2015
We discuss the generation of primary soft tissue sarcomas in mice using the Cre-loxP system to activate conditional mutations in oncogenic Kras and the tumor suppressor p53 (LSL-Kras(G12D/+); p53(flox/flox)). Sarcomas can be generated either by adenoviral delivery of Cre recombinase, activation of transgenic Cre recombinase with tamoxifen, or through transplantation of isolated satellite cells with Cre activation in vitro. Various applications of these models are discussed, including anticancer therapies, metastasis, in vivo imaging, and genetic requirements for tumorigenesis.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2015
Volume
1267
Start / End Page
283 / 295
Location
United States
Related Subject Headings
- Tumor Suppressor Protein p53
- Tamoxifen
- Sarcoma
- Proto-Oncogene Proteins p21(ras)
- Mutation
- Mice, Transgenic
- Mice
- Integrases
- Genetic Engineering
- Disease Models, Animal
Citation
APA
Chicago
ICMJE
MLA
NLM
Dodd, R. D., Añó, L., Blum, J. M., Li, Z., Van Mater, D., & Kirsch, D. G. (2015). Methods to generate genetically engineered mouse models of soft tissue sarcoma. Methods Mol Biol, 1267, 283–295. https://doi.org/10.1007/978-1-4939-2297-0_13
Dodd, Rebecca D., Leonor Añó, Jordan M. Blum, Zhizhong Li, David Van Mater, and David G. Kirsch. “Methods to generate genetically engineered mouse models of soft tissue sarcoma.” Methods Mol Biol 1267 (2015): 283–95. https://doi.org/10.1007/978-1-4939-2297-0_13.
Dodd RD, Añó L, Blum JM, Li Z, Van Mater D, Kirsch DG. Methods to generate genetically engineered mouse models of soft tissue sarcoma. Methods Mol Biol. 2015;1267:283–95.
Dodd, Rebecca D., et al. “Methods to generate genetically engineered mouse models of soft tissue sarcoma.” Methods Mol Biol, vol. 1267, 2015, pp. 283–95. Pubmed, doi:10.1007/978-1-4939-2297-0_13.
Dodd RD, Añó L, Blum JM, Li Z, Van Mater D, Kirsch DG. Methods to generate genetically engineered mouse models of soft tissue sarcoma. Methods Mol Biol. 2015;1267:283–295.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2015
Volume
1267
Start / End Page
283 / 295
Location
United States
Related Subject Headings
- Tumor Suppressor Protein p53
- Tamoxifen
- Sarcoma
- Proto-Oncogene Proteins p21(ras)
- Mutation
- Mice, Transgenic
- Mice
- Integrases
- Genetic Engineering
- Disease Models, Animal