Skip to main content

Pulse Field Gel Electrophoresis.

Publication ,  Journal Article
Sharma-Kuinkel, BK; Rude, TH; Fowler, VG
Published in: Methods Mol Biol
2016

Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction. PFGE is a variation of agarose gel electrophoresis that permits analysis of bacterial DNA fragments over an order of magnitude larger than that with conventional restriction enzyme analysis. It provides a good representation of the entire bacterial chromosome in a single gel with a highly reproducible restriction profile, providing clearly distinct and well-resolved DNA fragments.

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2016

Volume

1373

Start / End Page

117 / 130

Location

United States

Related Subject Headings

  • Genotype
  • Electrophoresis, Gel, Pulsed-Field
  • Developmental Biology
  • DNA, Bacterial
  • DNA Restriction Enzymes
  • DNA
  • Chromosomes, Bacterial
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sharma-Kuinkel, B. K., Rude, T. H., & Fowler, V. G. (2016). Pulse Field Gel Electrophoresis. Methods Mol Biol, 1373, 117–130. https://doi.org/10.1007/7651_2014_191
Sharma-Kuinkel, Batu K., Thomas H. Rude, and Vance G. Fowler. “Pulse Field Gel Electrophoresis.Methods Mol Biol 1373 (2016): 117–30. https://doi.org/10.1007/7651_2014_191.
Sharma-Kuinkel BK, Rude TH, Fowler VG. Pulse Field Gel Electrophoresis. Methods Mol Biol. 2016;1373:117–30.
Sharma-Kuinkel, Batu K., et al. “Pulse Field Gel Electrophoresis.Methods Mol Biol, vol. 1373, 2016, pp. 117–30. Pubmed, doi:10.1007/7651_2014_191.
Sharma-Kuinkel BK, Rude TH, Fowler VG. Pulse Field Gel Electrophoresis. Methods Mol Biol. 2016;1373:117–130.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2016

Volume

1373

Start / End Page

117 / 130

Location

United States

Related Subject Headings

  • Genotype
  • Electrophoresis, Gel, Pulsed-Field
  • Developmental Biology
  • DNA, Bacterial
  • DNA Restriction Enzymes
  • DNA
  • Chromosomes, Bacterial
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology