Skip to main content

Irisin - a myth rather than an exercise-inducible myokine.

Publication ,  Journal Article
Albrecht, E; Norheim, F; Thiede, B; Holen, T; Ohashi, T; Schering, L; Lee, S; Brenmoehl, J; Thomas, S; Drevon, CA; Erickson, HP; Maak, S
Published in: Sci Rep
March 9, 2015

The myokine irisin is supposed to be cleaved from a transmembrane precursor, FNDC5 (fibronectin type III domain containing 5), and to mediate beneficial effects of exercise on human metabolism. However, evidence for irisin circulating in blood is largely based on commercial ELISA kits which are based on polyclonal antibodies (pAbs) not previously tested for cross-reacting serum proteins. We have analyzed four commercial pAbs by Western blotting, which revealed prominent cross-reactivity with non-specific proteins in human and animal sera. Using recombinant glycosylated and non-glycosylated irisin as positive controls, we found no immune-reactive bands of the expected size in any biological samples. A FNDC5 signature was identified at ~20 kDa by mass spectrometry in human serum but was not detected by the commercial pAbs tested. Our results call into question all previous data obtained with commercial ELISA kits for irisin, and provide evidence against a physiological role for irisin in humans and other species.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

March 9, 2015

Volume

5

Start / End Page

8889

Location

England

Related Subject Headings

  • Sensitivity and Specificity
  • Reproducibility of Results
  • Muscle, Skeletal
  • Mice
  • Humans
  • Fibronectins
  • Exercise
  • Enzyme-Linked Immunosorbent Assay
  • Cytokines
  • Blood Chemical Analysis
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Albrecht, E., Norheim, F., Thiede, B., Holen, T., Ohashi, T., Schering, L., … Maak, S. (2015). Irisin - a myth rather than an exercise-inducible myokine. Sci Rep, 5, 8889. https://doi.org/10.1038/srep08889
Albrecht, Elke, Frode Norheim, Bernd Thiede, Torgeir Holen, Tomoo Ohashi, Lisa Schering, Sindre Lee, et al. “Irisin - a myth rather than an exercise-inducible myokine.Sci Rep 5 (March 9, 2015): 8889. https://doi.org/10.1038/srep08889.
Albrecht E, Norheim F, Thiede B, Holen T, Ohashi T, Schering L, et al. Irisin - a myth rather than an exercise-inducible myokine. Sci Rep. 2015 Mar 9;5:8889.
Albrecht, Elke, et al. “Irisin - a myth rather than an exercise-inducible myokine.Sci Rep, vol. 5, Mar. 2015, p. 8889. Pubmed, doi:10.1038/srep08889.
Albrecht E, Norheim F, Thiede B, Holen T, Ohashi T, Schering L, Lee S, Brenmoehl J, Thomas S, Drevon CA, Erickson HP, Maak S. Irisin - a myth rather than an exercise-inducible myokine. Sci Rep. 2015 Mar 9;5:8889.

Published In

Sci Rep

DOI

EISSN

2045-2322

Publication Date

March 9, 2015

Volume

5

Start / End Page

8889

Location

England

Related Subject Headings

  • Sensitivity and Specificity
  • Reproducibility of Results
  • Muscle, Skeletal
  • Mice
  • Humans
  • Fibronectins
  • Exercise
  • Enzyme-Linked Immunosorbent Assay
  • Cytokines
  • Blood Chemical Analysis