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Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis.

Publication ,  Journal Article
Raposo, AASF; Vasconcelos, FF; Drechsel, D; Marie, C; Johnston, C; Dolle, D; Bithell, A; Gillotin, S; van den Berg, DLC; Ettwiller, L ...
Published in: Cell Rep
March 10, 2015

The proneural transcription factor Ascl1 coordinates gene expression in both proliferating and differentiating progenitors along the neuronal lineage. Here, we used a cellular model of neurogenesis to investigate how Ascl1 interacts with the chromatin landscape to regulate gene expression when promoting neuronal differentiation. We find that Ascl1 binding occurs mostly at distal enhancers and is associated with activation of gene transcription. Surprisingly, the accessibility of Ascl1 to its binding sites in neural stem/progenitor cells remains largely unchanged throughout their differentiation, as Ascl1 targets regions of both readily accessible and closed chromatin in proliferating cells. Moreover, binding of Ascl1 often precedes an increase in chromatin accessibility and the appearance of new regions of open chromatin, associated with de novo gene expression during differentiation. Our results reveal a function of Ascl1 in promoting chromatin accessibility during neurogenesis, linking the chromatin landscape at Ascl1 target regions with the temporal progression of its transcriptional program.

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Published In

Cell Rep

DOI

EISSN

2211-1247

Publication Date

March 10, 2015

Volume

10

Issue

9

Start / End Page

1544 / 1556

Location

United States

Related Subject Headings

  • 31 Biological sciences
  • 1116 Medical Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Raposo, A. A. S. F., Vasconcelos, F. F., Drechsel, D., Marie, C., Johnston, C., Dolle, D., … Castro, D. S. (2015). Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis. Cell Rep, 10(9), 1544–1556. https://doi.org/10.1016/j.celrep.2015.02.025
Raposo, Alexandre A. S. F., Francisca F. Vasconcelos, Daniela Drechsel, Corentine Marie, Caroline Johnston, Dirk Dolle, Angela Bithell, et al. “Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis.Cell Rep 10, no. 9 (March 10, 2015): 1544–56. https://doi.org/10.1016/j.celrep.2015.02.025.
Raposo AASF, Vasconcelos FF, Drechsel D, Marie C, Johnston C, Dolle D, et al. Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis. Cell Rep. 2015 Mar 10;10(9):1544–56.
Raposo, Alexandre A. S. F., et al. “Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis.Cell Rep, vol. 10, no. 9, Mar. 2015, pp. 1544–56. Pubmed, doi:10.1016/j.celrep.2015.02.025.
Raposo AASF, Vasconcelos FF, Drechsel D, Marie C, Johnston C, Dolle D, Bithell A, Gillotin S, van den Berg DLC, Ettwiller L, Flicek P, Crawford GE, Parras CM, Berninger B, Buckley NJ, Guillemot F, Castro DS. Ascl1 Coordinately Regulates Gene Expression and the Chromatin Landscape during Neurogenesis. Cell Rep. 2015 Mar 10;10(9):1544–1556.
Journal cover image

Published In

Cell Rep

DOI

EISSN

2211-1247

Publication Date

March 10, 2015

Volume

10

Issue

9

Start / End Page

1544 / 1556

Location

United States

Related Subject Headings

  • 31 Biological sciences
  • 1116 Medical Physiology
  • 0601 Biochemistry and Cell Biology