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c-FLIP protects T lymphocytes from apoptosis in the intrinsic pathway.

Publication ,  Journal Article
He, M-X; He, Y-W
Published in: J Immunol
April 1, 2015

Apoptosis can be induced by either death receptors on the plasma membrane (extrinsic pathway) or the damage of the genome and/or cellular organelles (intrinsic pathway). Previous studies suggest that cellular caspase 8 (FLICE)-like inhibitory protein (c-FLIP) promotes cell survival in death receptor-induced apoptosis pathway in T lymphocytes. Independent of death receptor signaling, mitochondria sense apoptotic stimuli and mediate the activation of effector caspases. Whether c-FLIP regulates mitochondrion-dependent apoptotic signals remains unknown. In this study, c-FLIP gene was deleted in mature T lymphocytes in vitro, and the role of c-FLIP protein in intrinsic apoptosis pathway was studied. In resting T cells treated with the intrinsic apoptosis inducer, c-FLIP suppressed cytochrome c release from mitochondria. Bim-deletion rescued the enhanced apoptosis in c-FLIP-deficient T cells, whereas inhibition of caspase 8 did not. Different from activated T cells, there was no necroptosis or increase in reactive oxygen species in c-FLIP-deficient resting T cells. These data suggest that c-FLIP is a negative regulator of intrinsic apoptosis pathway in T lymphocytes.

Duke Scholars

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

April 1, 2015

Volume

194

Issue

7

Start / End Page

3444 / 3451

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Staurosporine
  • Reactive Oxygen Species
  • Proto-Oncogene Proteins
  • Mitochondria
  • Mice, Knockout
  • Mice
  • Membrane Proteins
  • Intracellular Space
  • Immunology
 

Citation

APA
Chicago
ICMJE
MLA
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He, M.-X., & He, Y.-W. (2015). c-FLIP protects T lymphocytes from apoptosis in the intrinsic pathway. J Immunol, 194(7), 3444–3451. https://doi.org/10.4049/jimmunol.1400469
He, Ming-Xiao, and You-Wen He. “c-FLIP protects T lymphocytes from apoptosis in the intrinsic pathway.J Immunol 194, no. 7 (April 1, 2015): 3444–51. https://doi.org/10.4049/jimmunol.1400469.
He M-X, He Y-W. c-FLIP protects T lymphocytes from apoptosis in the intrinsic pathway. J Immunol. 2015 Apr 1;194(7):3444–51.
He, Ming-Xiao, and You-Wen He. “c-FLIP protects T lymphocytes from apoptosis in the intrinsic pathway.J Immunol, vol. 194, no. 7, Apr. 2015, pp. 3444–51. Pubmed, doi:10.4049/jimmunol.1400469.
He M-X, He Y-W. c-FLIP protects T lymphocytes from apoptosis in the intrinsic pathway. J Immunol. 2015 Apr 1;194(7):3444–3451.

Published In

J Immunol

DOI

EISSN

1550-6606

Publication Date

April 1, 2015

Volume

194

Issue

7

Start / End Page

3444 / 3451

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Staurosporine
  • Reactive Oxygen Species
  • Proto-Oncogene Proteins
  • Mitochondria
  • Mice, Knockout
  • Mice
  • Membrane Proteins
  • Intracellular Space
  • Immunology