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Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China.

Publication ,  Journal Article
Chen, Y; Li, N; Zhang, T; Huang, X; Cai, F; Vandergrift, N; Xin, R; Meng, Z; Zhang, X; Jiang, C; Xu, X; Montefiori, DC; Gao, F; Wu, H
Published in: J Acquir Immune Defic Syndr
August 1, 2015

BACKGROUND: The men having sex with men (MSM) population has become one of the major risk groups for HIV-1 infection in China. However, the epidemiological patterns, function of the env genes, and autologous and heterologous neutralization activity in the same MSM population have not been systematically characterized. METHODS: The env gene sequences were obtained by the single genome amplification. The time to the most recent common ancestor was estimated for each genotype using the Bayesian Markov Chain Monte Carlo approach. Coreceptor usage was determined in NP-2 cells. Neutralization was analyzed using Env pseudoviruses in TZM-bl cells. RESULTS: We have obtained 547 full-length env gene sequences by single genome amplification from 30 acute/early HIV-1--infected individuals in the Beijing MSM cohort. Three genotypes (subtype B, CRF01_AE, and CRF07_BC) were identified and 20% of the individuals were infected with multiple transmitted/founder (T/F) viruses. The tight clusters of the MSM sequences regardless of geographic origins indicated nearly exclusive transmission within the MSM population and limited number of introductions. The time to the most recent common ancestor for each genotype was 10-15 years after each was first introduced in China. Disparate preferences for coreceptor usages among 3 genotypes might lead to the changes in percentage of different genotypes in the MSM population over time. The genotype-matched and genotype-mismatched neutralization activity varied among the 3 genotypes. CONCLUSIONS: The identification of unique characteristics for transmission, coreceptor usage, neutralization profile, and epidemic patterns of HIV-1 is critical for the better understanding of transmission mechanisms, development of preventive strategies, and evaluation of vaccine efficacy in the MSM population in China.

Duke Scholars

Published In

J Acquir Immune Defic Syndr

DOI

EISSN

1944-7884

Publication Date

August 1, 2015

Volume

69

Issue

4

Start / End Page

403 / 412

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Male
  • Humans
  • Homosexuality, Male
  • HIV-1
  • HIV Infections
  • Genotype
  • Gene Expression Regulation, Viral
  • China
 

Citation

APA
Chicago
ICMJE
MLA
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Chen, Y., Li, N., Zhang, T., Huang, X., Cai, F., Vandergrift, N., … Wu, H. (2015). Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China. J Acquir Immune Defic Syndr, 69(4), 403–412. https://doi.org/10.1097/QAI.0000000000000649
Chen, Yue, Ning Li, Tong Zhang, Xiaojie Huang, Fangping Cai, Nathan Vandergrift, Ruolei Xin, et al. “Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China.J Acquir Immune Defic Syndr 69, no. 4 (August 1, 2015): 403–12. https://doi.org/10.1097/QAI.0000000000000649.
Chen Y, Li N, Zhang T, Huang X, Cai F, Vandergrift N, et al. Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China. J Acquir Immune Defic Syndr. 2015 Aug 1;69(4):403–12.
Chen, Yue, et al. “Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China.J Acquir Immune Defic Syndr, vol. 69, no. 4, Aug. 2015, pp. 403–12. Pubmed, doi:10.1097/QAI.0000000000000649.
Chen Y, Li N, Zhang T, Huang X, Cai F, Vandergrift N, Xin R, Meng Z, Zhang X, Jiang C, Xu X, Montefiori DC, Gao F, Wu H. Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China. J Acquir Immune Defic Syndr. 2015 Aug 1;69(4):403–412.

Published In

J Acquir Immune Defic Syndr

DOI

EISSN

1944-7884

Publication Date

August 1, 2015

Volume

69

Issue

4

Start / End Page

403 / 412

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Virology
  • Male
  • Humans
  • Homosexuality, Male
  • HIV-1
  • HIV Infections
  • Genotype
  • Gene Expression Regulation, Viral
  • China